Expressions of stemness markers in keloid tissue

Sri S. Ningsih; Dewi H. Sari; Radiana D. Antarianto; Novi S. Hardiany; Mohamad Sadikin; Septelia I. Wanandi; Sri W.A. Jusman

doi:10.13181/mji.v27i3.1920

Expressions of stemness markers in keloid tissue

Sri S. Ningsih, Dewi H. Sari, Radiana D. Antarianto, Novi S. Hardiany, Mohamad Sadikin, Septelia I. Wanandi, Sri W.A. Jusman

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Background: Keloid is an abnormal wound healing process that extends beyond the site of injury. Keloid and tumor’s shared similarity of recurrence suggesting a shared underlying mechanism that involves stemness. Octamer-binding transcription factor-4 (Oct-4) and aldehyde dehydrogenase-1 (ALDH1) are stem cell stemness markers. This study aimed to analyze Oct-4 and ALDH1 expressions in keloid tissues. Methods: Samples were obtained from keloid tissue excisions from three keloid patients and post-circumcision preputial skin from three healthy donors (normal control) in accordance with the local ethical committee regulation. Total RNA was isolated using TriPure Isolation kit (Ameritech), and expressions of Oct4 and ALDH1 mRNA in keloid and preputial skin were determined by quantitative reverse transcription–polymerase chain reaction (qRT-PCR) using Livak method. Results: The qRT-PCR analysis revealed the expressions of Oct4 and ALDH1 in keloid and preputial skin tissues. Keloid tissues exhibited lower expression levels of Oct-4 and ALDH1 than the preputial skin. The difference was statistically insignificant. Conclusion: Keloid tissues express Oct-4 and ALDH1 as stemness markers, and the stemness characteristics of keloid might be similar to a normal skin.

Original language	English
Pages (from-to)	145-149
Number of pages	5
Journal	Medical Journal of Indonesia
Volume	27
Issue number	3
DOIs	https://doi.org/10.13181/mji.v27i3.1920
Publication status	Published - Sept 2018

Keywords

ALDH1
Keloid
Oct-4
Stemness

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@article{279f3006f5f640cf8314d34b048119af,

title = "Expressions of stemness markers in keloid tissue",

abstract = "Background: Keloid is an abnormal wound healing process that extends beyond the site of injury. Keloid and tumor{\textquoteright}s shared similarity of recurrence suggesting a shared underlying mechanism that involves stemness. Octamer-binding transcription factor-4 (Oct-4) and aldehyde dehydrogenase-1 (ALDH1) are stem cell stemness markers. This study aimed to analyze Oct-4 and ALDH1 expressions in keloid tissues. Methods: Samples were obtained from keloid tissue excisions from three keloid patients and post-circumcision preputial skin from three healthy donors (normal control) in accordance with the local ethical committee regulation. Total RNA was isolated using TriPure Isolation kit (Ameritech), and expressions of Oct4 and ALDH1 mRNA in keloid and preputial skin were determined by quantitative reverse transcription–polymerase chain reaction (qRT-PCR) using Livak method. Results: The qRT-PCR analysis revealed the expressions of Oct4 and ALDH1 in keloid and preputial skin tissues. Keloid tissues exhibited lower expression levels of Oct-4 and ALDH1 than the preputial skin. The difference was statistically insignificant. Conclusion: Keloid tissues express Oct-4 and ALDH1 as stemness markers, and the stemness characteristics of keloid might be similar to a normal skin.",

keywords = "ALDH1, Keloid, Oct-4, Stemness",

author = "Ningsih, {Sri S.} and Sari, {Dewi H.} and Antarianto, {Radiana D.} and Hardiany, {Novi S.} and Mohamad Sadikin and Wanandi, {Septelia I.} and Jusman, {Sri W.A.}",

note = "Funding Information: Researchers would like to express their gratitude to Direktorat Riset dan Pengabdian Masyarakat UI, who funded this research through Hibah Publikasi Il?iah Terindeks Tugas Akhir (PITTA). Publisher Copyright: {\textcopyright} 2018 Authors.",

year = "2018",

month = sep,

doi = "10.13181/mji.v27i3.1920",

language = "English",

volume = "27",

pages = "145--149",

journal = "Medical Journal of Indonesia",

issn = "0853-1773",

publisher = "Faculty of Medicine Universitas Indonesia",

number = "3",

}

TY - JOUR

T1 - Expressions of stemness markers in keloid tissue

AU - Ningsih, Sri S.

AU - Sari, Dewi H.

AU - Antarianto, Radiana D.

AU - Hardiany, Novi S.

AU - Sadikin, Mohamad

AU - Wanandi, Septelia I.

AU - Jusman, Sri W.A.

N1 - Funding Information: Researchers would like to express their gratitude to Direktorat Riset dan Pengabdian Masyarakat UI, who funded this research through Hibah Publikasi Il?iah Terindeks Tugas Akhir (PITTA). Publisher Copyright: © 2018 Authors.

PY - 2018/9

Y1 - 2018/9

N2 - Background: Keloid is an abnormal wound healing process that extends beyond the site of injury. Keloid and tumor’s shared similarity of recurrence suggesting a shared underlying mechanism that involves stemness. Octamer-binding transcription factor-4 (Oct-4) and aldehyde dehydrogenase-1 (ALDH1) are stem cell stemness markers. This study aimed to analyze Oct-4 and ALDH1 expressions in keloid tissues. Methods: Samples were obtained from keloid tissue excisions from three keloid patients and post-circumcision preputial skin from three healthy donors (normal control) in accordance with the local ethical committee regulation. Total RNA was isolated using TriPure Isolation kit (Ameritech), and expressions of Oct4 and ALDH1 mRNA in keloid and preputial skin were determined by quantitative reverse transcription–polymerase chain reaction (qRT-PCR) using Livak method. Results: The qRT-PCR analysis revealed the expressions of Oct4 and ALDH1 in keloid and preputial skin tissues. Keloid tissues exhibited lower expression levels of Oct-4 and ALDH1 than the preputial skin. The difference was statistically insignificant. Conclusion: Keloid tissues express Oct-4 and ALDH1 as stemness markers, and the stemness characteristics of keloid might be similar to a normal skin.

AB - Background: Keloid is an abnormal wound healing process that extends beyond the site of injury. Keloid and tumor’s shared similarity of recurrence suggesting a shared underlying mechanism that involves stemness. Octamer-binding transcription factor-4 (Oct-4) and aldehyde dehydrogenase-1 (ALDH1) are stem cell stemness markers. This study aimed to analyze Oct-4 and ALDH1 expressions in keloid tissues. Methods: Samples were obtained from keloid tissue excisions from three keloid patients and post-circumcision preputial skin from three healthy donors (normal control) in accordance with the local ethical committee regulation. Total RNA was isolated using TriPure Isolation kit (Ameritech), and expressions of Oct4 and ALDH1 mRNA in keloid and preputial skin were determined by quantitative reverse transcription–polymerase chain reaction (qRT-PCR) using Livak method. Results: The qRT-PCR analysis revealed the expressions of Oct4 and ALDH1 in keloid and preputial skin tissues. Keloid tissues exhibited lower expression levels of Oct-4 and ALDH1 than the preputial skin. The difference was statistically insignificant. Conclusion: Keloid tissues express Oct-4 and ALDH1 as stemness markers, and the stemness characteristics of keloid might be similar to a normal skin.

KW - ALDH1

KW - Keloid

KW - Oct-4

KW - Stemness

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DO - 10.13181/mji.v27i3.1920

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AN - SCOPUS:85055188435

SN - 0853-1773

VL - 27

SP - 145

EP - 149

JO - Medical Journal of Indonesia

JF - Medical Journal of Indonesia

IS - 3

ER -

Expressions of stemness markers in keloid tissue

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