TY - JOUR
T1 - Expression, purification, crystallization and preliminary crystallographic analysis of human Rad GTPase
AU - Yanuar, Arry
AU - Sakurai, Shigeru
AU - Kitano, Ken
AU - Hakoshima, Toshio
PY - 2005
Y1 - 2005
N2 - Human Rad is a new member of the Ras GTPase superfamily and is overexpressed in human skeletal muscle of individuals with type II diabetes. The GTPase core domain was overexpressed in Escherichia coli and purified for crystallization. Crystals were obtained at 293 K by vapour diffusion using a crystallization robot. The crystals were found to belong to space group P2 1, with unit-cell parameters a = 52.2, b = 58.6, c = 53.4 Å, β = 97.9°, and contained two Rad molecules in the crystallographic asymmetric unit. A diffraction data set was collected to a resolution of 1.8 Å using synchrotron radiation at SPring-8.
AB - Human Rad is a new member of the Ras GTPase superfamily and is overexpressed in human skeletal muscle of individuals with type II diabetes. The GTPase core domain was overexpressed in Escherichia coli and purified for crystallization. Crystals were obtained at 293 K by vapour diffusion using a crystallization robot. The crystals were found to belong to space group P2 1, with unit-cell parameters a = 52.2, b = 58.6, c = 53.4 Å, β = 97.9°, and contained two Rad molecules in the crystallographic asymmetric unit. A diffraction data set was collected to a resolution of 1.8 Å using synchrotron radiation at SPring-8.
UR - http://www.scopus.com/inward/record.url?scp=33744470113&partnerID=8YFLogxK
U2 - 10.1107/S1744309105031982
DO - 10.1107/S1744309105031982
M3 - Article
C2 - 16511212
AN - SCOPUS:33744470113
SN - 1744-3091
VL - 61
SP - 978
EP - 980
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 11
ER -