Expression, purification, crystallization and preliminary crystallographic analysis of human Rad GTPase

Arry Yanuar, Shigeru Sakurai, Ken Kitano, Toshio Hakoshima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human Rad is a new member of the Ras GTPase superfamily and is overexpressed in human skeletal muscle of individuals with type II diabetes. The GTPase core domain was overexpressed in Escherichia coli and purified for crystallization. Crystals were obtained at 293 K by vapour diffusion using a crystallization robot. The crystals were found to belong to space group P2 1, with unit-cell parameters a = 52.2, b = 58.6, c = 53.4 Å, β = 97.9°, and contained two Rad molecules in the crystallographic asymmetric unit. A diffraction data set was collected to a resolution of 1.8 Å using synchrotron radiation at SPring-8.

Original languageEnglish
Pages (from-to)978-980
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number11
DOIs
Publication statusPublished - 1 Dec 2005

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