TY - JOUR
T1 - Expression and role of HIF-1α and HIF-2α in tissue regeneration
T2 - a study of hypoxia in house gecko tail regeneration
AU - Novianti, Titta
AU - Juniantito, Vetnizah
AU - Jusuf, Ahmad Aulia
AU - Arida, Evy Ayu
AU - Jusman, Sri Widia A.
AU - Sadikin, Mohamad
N1 - Funding Information:
This work was supported by the Universitas Indonesia [1314/UN 2.R3.1/HKP.05.00/2018]. We would like to thank to the University of Indonesia for funding this research (Universitas Indonesia (UI) Research Grant no. and Doctoral Final Assignment Grant No. 1314/UN 2.R3.1/HKP.05.00/2018). We would also like to thank Ecosains Company for the chance to use a real-time qPCR Eco machine. Our gratitude is also owed to the Center for Hypoxia and Oxidative Stress in the Biochemistry Department in the Faculty of Medicine at the University of Indonesia and the Indonesian Institute of Sciences (LIPI) for the opportunity to use their laboratory during our research, as well as to the Research Center for Virus and Cancer Biology (PRVKP) at the University of Indonesia. Finally, we would also like to thank the LIPI researchers Dr. Marlina and Dr. Amir, who helped with the design of the H. platyurus DNA primer.
Funding Information:
We would like to thank to the University of Indonesia for funding this research (Universitas Indonesia (UI) Research Grant no. and Doctoral Final Assignment Grant No. 1314/UN 2.R3.1/HKP.05.00/2018). We would also like to thank Ecosains Company for the chance to use a real-time qPCR Eco machine. Our gratitude is also owed to the Center for Hypoxia and Oxidative Stress in the Biochemistry Department in the Faculty of Medicine at the University of Indonesia and the Indonesian Institute of Sciences (LIPI) for the opportunity to use their laboratory during our research, as well as to the Research Center for Virus and Cancer Biology (PRVKP) at the University of Indonesia. Finally, we would also like to thank the LIPI researchers Dr. Marlina and Dr. Amir, who helped with the design of the H. platyurus DNA primer. Krafts KP. The hidden drama tissue repair. Organogenesis . 2010 ; 6 ( 4 ): 225 – 33 . doi: 10.4161/org6.4.12555 . Joven A , Simon A.
Publisher Copyright:
© 2019, © 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - The house gecko (Hemidactylus platyurus) has evolved the ability to autotomize its tail when threatened. The lost part is then regrown via epimorphic regeneration in a process that requires high energy and oxygen levels. Oxygen demand is therefore likely to outstrip supply and this can result in relative hypoxia in the tissues of the regenerating tail. The hypoxic state is stabilized by the Hypoxia Inducible Factor-1α (HIF-1α) and HIF-2α proteins. We induced tail autotomy in 30 mal H. platyurus adults using a standard procedure and then collected samples of the regenerated tail tissue on days 1, 3, 5, 8, 10, 13, 17, 21, 25, and 30 post autotomy. For each sample, mRNA expression was analyzed by qPCR, proteins were analyzed using Western Blot tests and immunohistochemistry, and the histological structure was analyzed using Hematoxylin and Eosin staining. On day 1, HIF-1α mRNA expression increased and the tissue was dominated by leucocyte and erythrocyte cells. HIF-1α mRNA expression peaked on day 3, at which time some cells were actively proliferating, migrating, and differentiating. At the same time as HIF-1α expression decreased, HIF-2α mRNA expression increased, as did overall cellular activity. HIF-2α expression increased more gradually but was present over a longer period of time than HIF-1α. We hypothesize that HIF-1α helps to initially stimulate the tissue regeneration process while HIF-2α functionally takes over the role of HIF-1α after HIF-1α succumbs to the oxygen conditions, but we suspect that both HIF-1α and HIF-2α play a role in overcoming the tissue’s hypoxic state.
AB - The house gecko (Hemidactylus platyurus) has evolved the ability to autotomize its tail when threatened. The lost part is then regrown via epimorphic regeneration in a process that requires high energy and oxygen levels. Oxygen demand is therefore likely to outstrip supply and this can result in relative hypoxia in the tissues of the regenerating tail. The hypoxic state is stabilized by the Hypoxia Inducible Factor-1α (HIF-1α) and HIF-2α proteins. We induced tail autotomy in 30 mal H. platyurus adults using a standard procedure and then collected samples of the regenerated tail tissue on days 1, 3, 5, 8, 10, 13, 17, 21, 25, and 30 post autotomy. For each sample, mRNA expression was analyzed by qPCR, proteins were analyzed using Western Blot tests and immunohistochemistry, and the histological structure was analyzed using Hematoxylin and Eosin staining. On day 1, HIF-1α mRNA expression increased and the tissue was dominated by leucocyte and erythrocyte cells. HIF-1α mRNA expression peaked on day 3, at which time some cells were actively proliferating, migrating, and differentiating. At the same time as HIF-1α expression decreased, HIF-2α mRNA expression increased, as did overall cellular activity. HIF-2α expression increased more gradually but was present over a longer period of time than HIF-1α. We hypothesize that HIF-1α helps to initially stimulate the tissue regeneration process while HIF-2α functionally takes over the role of HIF-1α after HIF-1α succumbs to the oxygen conditions, but we suspect that both HIF-1α and HIF-2α play a role in overcoming the tissue’s hypoxic state.
KW - differentiation
KW - HIF
KW - house gecko
KW - hypoxia
KW - proliferation
UR - http://www.scopus.com/inward/record.url?scp=85070887780&partnerID=8YFLogxK
U2 - 10.1080/15476278.2019.1644889
DO - 10.1080/15476278.2019.1644889
M3 - Article
AN - SCOPUS:85070887780
VL - 15
SP - 69
EP - 84
JO - Organogenesis
JF - Organogenesis
SN - 1547-6278
IS - 3
ER -