Exposure of gel emulsion zoledronate bisphosphonate olive oil increase osteoclast apoptosis

Several researchers reported that injection of Zoledronate Bisphosphonate (ZOL) could inhibit orthodontic tooth movement by osteoclasts apoptosis, but the administration by injection is painless. Use of ZOL emulsion with olive oil in a gel (Ge-ZOL) is expected to overcome the lack of administration by injection. To proved the effects of exposure of Ge-ZOL with olive oil on the number of osteoclasts apoptosis. This research was an in vivo laboratory experiment using 27 Sprague Dawley rats consist of 9 rats experimental group received Ge-ZOL, 9 rats control group receive Ge-without ZOL and 9 rats of normal groups. Gel with 40 μg of ZOL and gel without ZOL were applied on oral mucosa on beginning, hours to four to eight for two minutes on days 1, 2, 3 and 4. Rats were sacrificed on days 1, 3, 5 and then made preparation histological staining with IHK Caspase-3. Ge-ZOL could increase the number of osteoclasts apoptosis on day 1 and 3 as well as the highest increase on the 3rd day. Ge-ZOL with olive oil dose of 40 μg proven to increase the number of osteoclast apoptosis.