Knowledge management is a universal need for all organizations, and ministerial military defense organization is no exception. Some studies have described the KM implementation in various types of organizations. However, the study that comprehensively explores the KM implementation in military organizations is still deemed lacking. Intriguingly, different cultures in a military defense organization that consisted of both military and civilian personnel may affect the perception and the implementation of KM in a unique way. This study investigated the current state of KM implementation in a ministerial defense research and development agency and its personnel perceptions regarding the definition and the impacts of KM. Semi-structured interviews across commanding officers, military personnel, and civilian cohorts were conducted to gain some insights about the KM implementation. The results showed that KM practices had been implemented in a limited way, with a heavy emphasis on knowledge internalization and sharing. Finally, some recommendations were proposed based on the findings to cater to the needs of the organization.