TY - JOUR
T1 - Exploration of anthelmintic activity of Cassia spp. extracts on gastrointestinal nematodes of sheep
AU - Wahyuni, Sri
AU - Sunarso, Sunarso
AU - Prasetiyono, Bambang Waluyo Hadi Eko
AU - Satrija, Fadjar
N1 - Funding Information:
This research was 퀀inanced by the Doctoral Dissertation Research Grant of the Ministry of Research, Technology and Higher Education of Republic of Indonesian through Domestic Graduate Scholarship (“BPP-DN”) program. The authors would like to thank Mr. Sulaiman and Department Animal Infectious Diseases, and Veterinary Public Health, Faculty Veterinary Medicine, IPB University, Bogor for providing laboratory facilities during the study.
Publisher Copyright:
© 2019, Journal of Advanced Veterinary and Animal Research.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Objective: This study aimed to explore the phytochemical constituents and anthelmintic activities of four Cassia spp. leaves against Haemonchus contortus. Materials and Methods: The extracts were prepared from four species of Cassia spp. (C. siamea, C. fistula, C. surattensis, and C. spectabilis). Phytochemical screening of the extract was done based on the Harborne method. Evaluation of the anthelmintic activities against H. contortus was done in vitro using infective larvae (L3) migration inhibition assay (LMIA). Measurement of larvae migrating was conducted through a nylon filter with a pore size of 20 μm. The doses of Cassia spp. extract implemented were 25, 50, 100, and 200 mg/ml. Results: Tannins, alkaloids, phenol hydroquinone, flavonoids, steroids, triterpenoids, and saponins were present in all the extracts, whereas alkaloids were absent in C. fistula. No triterpenoids were found in C. surattensis and C. spectabilis. Movement of H. contortus larvae was significantly inhibited after exposure to Cassia extracts at various dosage levels (p < 0.05). The test results using LMIA on L3 H. contortus showed the lowest inhibition in the negative control. Among the species of Cassia, the C. surattensis (at 200 mg/ml) showed the highest (p < 0.05) inhibition level on the larvae. The latter result corresponded to the effect of albendazole. Conclusion: Compared to other Cassia spp., C. surattensis exhibited the highest inhibition against L3 H. contortus. However, the inhibition effect of C. surattensis was still lower as compared to albendazole.
AB - Objective: This study aimed to explore the phytochemical constituents and anthelmintic activities of four Cassia spp. leaves against Haemonchus contortus. Materials and Methods: The extracts were prepared from four species of Cassia spp. (C. siamea, C. fistula, C. surattensis, and C. spectabilis). Phytochemical screening of the extract was done based on the Harborne method. Evaluation of the anthelmintic activities against H. contortus was done in vitro using infective larvae (L3) migration inhibition assay (LMIA). Measurement of larvae migrating was conducted through a nylon filter with a pore size of 20 μm. The doses of Cassia spp. extract implemented were 25, 50, 100, and 200 mg/ml. Results: Tannins, alkaloids, phenol hydroquinone, flavonoids, steroids, triterpenoids, and saponins were present in all the extracts, whereas alkaloids were absent in C. fistula. No triterpenoids were found in C. surattensis and C. spectabilis. Movement of H. contortus larvae was significantly inhibited after exposure to Cassia extracts at various dosage levels (p < 0.05). The test results using LMIA on L3 H. contortus showed the lowest inhibition in the negative control. Among the species of Cassia, the C. surattensis (at 200 mg/ml) showed the highest (p < 0.05) inhibition level on the larvae. The latter result corresponded to the effect of albendazole. Conclusion: Compared to other Cassia spp., C. surattensis exhibited the highest inhibition against L3 H. contortus. However, the inhibition effect of C. surattensis was still lower as compared to albendazole.
KW - Anthelmintic effect
KW - C. fistula
KW - C. siamea
KW - C. spectabilis
KW - C. surattensis
KW - H. contortus
UR - http://www.scopus.com/inward/record.url?scp=85067601537&partnerID=8YFLogxK
U2 - 10.5455/javar.2019.f338
DO - 10.5455/javar.2019.f338
M3 - Article
AN - SCOPUS:85067601537
SN - 2311-7710
VL - 6
SP - 236
EP - 240
JO - Journal of Advanced Veterinary and Animal Research
JF - Journal of Advanced Veterinary and Animal Research
IS - 2
ER -