Experimental Study of Tumescence Injection to Provide Anesthesia Without Local Anesthetics

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: One-per-mil tumescent technique could provide local anesthesia in surgical procedures despite using low lidocaine concentration. Tumescence environment within the tissue may play a role in supporting local anesthesia effect. This study aims to delineate whether the tumescence environment, created by one-per-mil solution without a local anesthetic agent, had a local anesthesia effect. Methods: Four different compositions and volumes of tumescent solutions containing 1:1,000,000 epinephrine were injected into 50 Swiss Webster mice. The animals were divided into five groups: group A, a tumescent solution with 0.2% lidocaine; group B, a tumescent solution with 0.04% lidocaine; group C, a tumescent solution without lidocaine; group D, a doubled volume of tumescent solution without lidocaine; and group E, controls. Local anesthesia effects were tested by using the formalin test (n = 25) and tail immersion test (n = 25). Pain response behavior in the form of paw licking duration and tail withdrawal latency was observed. The analysis of variance and Kruskal-Wallis tests were used to test the statistical difference. Significance was set at P < 0.05. Results: The four interventional groups showed less pain response behavior and significantly longer tail withdrawal latency (P < 0.05) than the control group. However, the groups showed nonsignificantly shorter paw licking duration than the control (P > 0.05). Group A had the fastest onset of the local anesthesia effect. Conclusions: The tumescence environment was potentially able to provide a local anesthesia effect, although the solution did not contain a local anesthetic agent as proved by the tail immersion test.

Original languageEnglish
Pages (from-to)295-301
Number of pages7
JournalJournal of Surgical Research
Volume245
DOIs
Publication statusPublished - 1 Jan 2020

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Local Anesthetics
Local Anesthesia
Anesthesia
Lidocaine
Injections
Tail
Immersion
Anesthetics
Pain Measurement
Epinephrine
Analysis of Variance
Pain
Control Groups

Keywords

  • Anesthesia
  • Edema
  • Hand
  • Lidocaine
  • Local
  • Pain

Cite this

@article{aaf5744a9fe54d4eb3653101baa9aca2,
title = "Experimental Study of Tumescence Injection to Provide Anesthesia Without Local Anesthetics",
abstract = "Background: One-per-mil tumescent technique could provide local anesthesia in surgical procedures despite using low lidocaine concentration. Tumescence environment within the tissue may play a role in supporting local anesthesia effect. This study aims to delineate whether the tumescence environment, created by one-per-mil solution without a local anesthetic agent, had a local anesthesia effect. Methods: Four different compositions and volumes of tumescent solutions containing 1:1,000,000 epinephrine were injected into 50 Swiss Webster mice. The animals were divided into five groups: group A, a tumescent solution with 0.2{\%} lidocaine; group B, a tumescent solution with 0.04{\%} lidocaine; group C, a tumescent solution without lidocaine; group D, a doubled volume of tumescent solution without lidocaine; and group E, controls. Local anesthesia effects were tested by using the formalin test (n = 25) and tail immersion test (n = 25). Pain response behavior in the form of paw licking duration and tail withdrawal latency was observed. The analysis of variance and Kruskal-Wallis tests were used to test the statistical difference. Significance was set at P < 0.05. Results: The four interventional groups showed less pain response behavior and significantly longer tail withdrawal latency (P < 0.05) than the control group. However, the groups showed nonsignificantly shorter paw licking duration than the control (P > 0.05). Group A had the fastest onset of the local anesthesia effect. Conclusions: The tumescence environment was potentially able to provide a local anesthesia effect, although the solution did not contain a local anesthetic agent as proved by the tail immersion test.",
keywords = "Anesthesia, Edema, Hand, Lidocaine, Local, Pain",
author = "Biben, {Johannes A.} and Prasetyono, {Theddeus O.H.} and Tantri, {Aida R.}",
year = "2020",
month = "1",
day = "1",
doi = "10.1016/j.jss.2019.07.066",
language = "English",
volume = "245",
pages = "295--301",
journal = "Journal of Surgical Research",
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Experimental Study of Tumescence Injection to Provide Anesthesia Without Local Anesthetics. / Biben, Johannes A.; Prasetyono, Theddeus O.H.; Tantri, Aida R.

In: Journal of Surgical Research, Vol. 245, 01.01.2020, p. 295-301.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Experimental Study of Tumescence Injection to Provide Anesthesia Without Local Anesthetics

AU - Biben, Johannes A.

AU - Prasetyono, Theddeus O.H.

AU - Tantri, Aida R.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Background: One-per-mil tumescent technique could provide local anesthesia in surgical procedures despite using low lidocaine concentration. Tumescence environment within the tissue may play a role in supporting local anesthesia effect. This study aims to delineate whether the tumescence environment, created by one-per-mil solution without a local anesthetic agent, had a local anesthesia effect. Methods: Four different compositions and volumes of tumescent solutions containing 1:1,000,000 epinephrine were injected into 50 Swiss Webster mice. The animals were divided into five groups: group A, a tumescent solution with 0.2% lidocaine; group B, a tumescent solution with 0.04% lidocaine; group C, a tumescent solution without lidocaine; group D, a doubled volume of tumescent solution without lidocaine; and group E, controls. Local anesthesia effects were tested by using the formalin test (n = 25) and tail immersion test (n = 25). Pain response behavior in the form of paw licking duration and tail withdrawal latency was observed. The analysis of variance and Kruskal-Wallis tests were used to test the statistical difference. Significance was set at P < 0.05. Results: The four interventional groups showed less pain response behavior and significantly longer tail withdrawal latency (P < 0.05) than the control group. However, the groups showed nonsignificantly shorter paw licking duration than the control (P > 0.05). Group A had the fastest onset of the local anesthesia effect. Conclusions: The tumescence environment was potentially able to provide a local anesthesia effect, although the solution did not contain a local anesthetic agent as proved by the tail immersion test.

AB - Background: One-per-mil tumescent technique could provide local anesthesia in surgical procedures despite using low lidocaine concentration. Tumescence environment within the tissue may play a role in supporting local anesthesia effect. This study aims to delineate whether the tumescence environment, created by one-per-mil solution without a local anesthetic agent, had a local anesthesia effect. Methods: Four different compositions and volumes of tumescent solutions containing 1:1,000,000 epinephrine were injected into 50 Swiss Webster mice. The animals were divided into five groups: group A, a tumescent solution with 0.2% lidocaine; group B, a tumescent solution with 0.04% lidocaine; group C, a tumescent solution without lidocaine; group D, a doubled volume of tumescent solution without lidocaine; and group E, controls. Local anesthesia effects were tested by using the formalin test (n = 25) and tail immersion test (n = 25). Pain response behavior in the form of paw licking duration and tail withdrawal latency was observed. The analysis of variance and Kruskal-Wallis tests were used to test the statistical difference. Significance was set at P < 0.05. Results: The four interventional groups showed less pain response behavior and significantly longer tail withdrawal latency (P < 0.05) than the control group. However, the groups showed nonsignificantly shorter paw licking duration than the control (P > 0.05). Group A had the fastest onset of the local anesthesia effect. Conclusions: The tumescence environment was potentially able to provide a local anesthesia effect, although the solution did not contain a local anesthetic agent as proved by the tail immersion test.

KW - Anesthesia

KW - Edema

KW - Hand

KW - Lidocaine

KW - Local

KW - Pain

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DO - 10.1016/j.jss.2019.07.066

M3 - Article

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JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

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