Expansion Grade and Morphokinetic Parameters Associated with Aneuploidy Status of Embryo Fifth Day

Achmad K. Harzif, Muharam Natadisastra, Dwi A. Suryandari, Retno A. Werdhani, Wisnu Jatmiko, Arief Boediono, Hartanto Bayuaji, Budi Wiweko

Research output: Contribution to journalArticlepeer-review


Objective: To determine the potential of examining embryo morphology, and morphokinetic parameters in predicting the chromosomal status of embryos. Methods: This study is a cross-sectional that requires patients undergoing IVF followed by chromosome examination with NGS that was conducted at the IVF Center at Pondok Indah Hospital and Morula IVF Center at Bunda Hospital from December 2021 to December 2022. Each embryo that reaches the blastocyst stage on day 5 or 6 will be washed and put into a PCR tube for a week; then, embryologists annotate them to determine morphological assessment and morphokinetic parameters using Time-Lapse Microscopy. The chi-square test was used to analyse bivariate variables. Results: One hundred twenty-four samples were collected on day 5 of patients undergoing the IVF procedure. 50.8% of the samples were aneuploid chromosomes, and 49.2% were euploid. The morphokinetic characteristics median was 3.86 fold. It was found that expansion grade, time to pro-nuclear fading, and time to the synchrony of the third cell cycle were significantly associated with euploid status (p = 0.000; 0.041 and 0.036). Conclusion: The expansion grade has been proven as the most influential component for accurately predicting the ploidy status of embryos.

Original languageEnglish
Pages (from-to)220-227
Number of pages8
JournalIndonesian Journal of Obstetrics and Gynecology
Issue number4
Publication statusPublished - Oct 2023


  • blastocyst
  • embryo
  • euploid status
  • expansion grade
  • morphokinetics


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