In the use of a new technology in the form of an online laboratory, it is necessary to analyze factors affecting behavioral intentions to use online laboratory services in the health field. The purpose of this study is to analyze Technology, Personal, and Environment (TPE) to the adoption of online laboratory services. The model used in this study incorporates the TPE model that measures the acceptance of technology at the individual level with the theory of technology acceptance behavior in terms of attitude and intention of online laboratory users in the health field. The model was tested with SEM-PLS modeling by using SmartPLS3 software. The respondents in this study are Indonesian people who have not used an online laboratory and who have used an online laboratory to check his health as many as 76 people. The results of identification on the respondents indicated that for personal, the risk factors that would be obtained by the public did not affect their intention to adopt an online laboratory system while the old habit or behavior of society using conventional laboratory methods and perceived cost factors affected their intention to use a new online laboratory system. Other than that, all environmental factors had a significant positive effect on perceived usefulness unless the facilitating condition had no significant effect on perceived ease of use.