Nuclear medicine is one of medical fields that uses ionizing radiation sources for therapeutic and diagnostic imaging for cancer. The number of nuclear medicine services in Indonesia is 17 hospitals, which only 10 is active in conducting nuclear medicine services because it doesn't meet the requirements. Regulations about the standards of nuclear medicine services Indonesia are regulated by KMK number 008/MENKES/SK/I/2009 but it have not yet been discussed the requirements in details for building design and layout design as reference. For this reason, the author did study about the requirements of a nuclear medical service facility referring to KMK number 008/MENKES/SK/I/2009 and the International Health Facility Guidelines to create layout design of nuclear medicine facility. This study is done by comparing national and international regulation for planning criteria and construct layout design of nuclear medicine facility as recommendation to accomplish the regulations in Ministry of Health decree, Republic of Indonesia number 008/MENKES/SK/I/2009 regarding nuclear medical requirements.