TY - JOUR
T1 - Evaluation of the Stability and in Vitro Anti-inflammatory Activity of Partially Purified Bromelain Nanoemulsion
AU - Randy, Fransiskus
AU - Setiasih, Siswati
AU - Jufri, Mahdi
AU - Handayani, Sri
AU - Hudiyono, Sumi
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2020/6/30
Y1 - 2020/6/30
N2 - Bromelain is an enzyme belonging to cysteine protease. It is known for topical use as debridement for inflammation treatment. Nanoemulsion of bromelain (NEB) aims to solve this problem and also improve its stability. NEB was made by high emulsification method using homogenizer and sonicator. Nanoemulsion physicochemical stabilities were evaluated. The bromelain as an active compound was isolated from the core of pineapple (Ananas comosus [L.] Merr) which was purified by ammonium sulphate fractionation and then dialysis followed. Nanoemulsion formula 2 was found to be the most suitable formula for encapsulating bromelain with good physicochemical stability after 30 days of storage. This formula showed clear visual appearance with globule diameter of 37.42 nm with polydispersity index of 0.40. The result showed that the fraction of bromelain obtained from each purification step showed an increase in specific activity. The specific activities of fractionation using 20-50% ammonium sulphate and dialysis were 152.05 and 266.58 U/mg, respectively. The purest bromelain fraction was encapsulated into nanoemulsion formula 2. The bromelain fraction's anti-inflammatory activity was tested based on HRBC membrane stabilization method. The result showed that bromelain dialysis fraction shows the ability as anti-inflammatory agent with stability percentage of 79.76%.
AB - Bromelain is an enzyme belonging to cysteine protease. It is known for topical use as debridement for inflammation treatment. Nanoemulsion of bromelain (NEB) aims to solve this problem and also improve its stability. NEB was made by high emulsification method using homogenizer and sonicator. Nanoemulsion physicochemical stabilities were evaluated. The bromelain as an active compound was isolated from the core of pineapple (Ananas comosus [L.] Merr) which was purified by ammonium sulphate fractionation and then dialysis followed. Nanoemulsion formula 2 was found to be the most suitable formula for encapsulating bromelain with good physicochemical stability after 30 days of storage. This formula showed clear visual appearance with globule diameter of 37.42 nm with polydispersity index of 0.40. The result showed that the fraction of bromelain obtained from each purification step showed an increase in specific activity. The specific activities of fractionation using 20-50% ammonium sulphate and dialysis were 152.05 and 266.58 U/mg, respectively. The purest bromelain fraction was encapsulated into nanoemulsion formula 2. The bromelain fraction's anti-inflammatory activity was tested based on HRBC membrane stabilization method. The result showed that bromelain dialysis fraction shows the ability as anti-inflammatory agent with stability percentage of 79.76%.
UR - http://www.scopus.com/inward/record.url?scp=85087873141&partnerID=8YFLogxK
U2 - 10.1088/1757-899X/833/1/012038
DO - 10.1088/1757-899X/833/1/012038
M3 - Conference article
AN - SCOPUS:85087873141
SN - 1757-8981
VL - 833
JO - IOP Conference Series: Materials Science and Engineering
JF - IOP Conference Series: Materials Science and Engineering
IS - 1
M1 - 012038
T2 - 2nd International Conference on Chemistry and Material Science, IC2MS 2019
Y2 - 2 November 2019 through 3 November 2019
ER -