TY - GEN
T1 - Evaluation of the potential of Nano Silver Fluoride against Streptococcus mutans and Enterobacter faecalis in various stages of biofilm maturation
AU - Gultom, Ferry P.
AU - Khoirunnisa, Novia
AU - Sahlan, Muhamad
AU - Soekanto, Sri Angky
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/4/9
Y1 - 2019/4/9
N2 -
Silver Diamine Fluoride (SDF) is a liquid that has been proven to be an anti caries agent. Unfortunately, SDF leaves a black stain on the surface of the teeth where it was applicated, and it also has a metallic taste. Nano Silver Fluoride has the potential to replace Silver Diamine Fluoride as an anti caries agent without having the same side effects. To analyze the effect of NSF on the viability of Streptococcus mutans and Enterobacter faecalis in various phases of biofilm maturation compared to SDF, E. faecalis and S. mutans biofilm was incubated for 4 hours (adherence phase), 12 hours (active accumulation phase) and 24 hours (maturation phase) at 37 °C, then exposed to NSF at concentration (Ag
+
0,4%+F
-
2,26%), (Ag
+
0,9%+F
-
2,26%), (Ag
+
1,4%+F
-
2,26%), (Ag
+
1,9%+F
-
2,26%) for 1 hour. The percentage of viability was tested with MTT assay. Biofilm viability of E. faecalis and S. mutans in various phases showed no significant difference (p>0,05) in one-way ANOVA, compared to SDF. Results from this study showed that NSF was comparable with Silver Diamine Fluoride in inhibiting formation of E. faecalis and S. mutans biofilm.
AB -
Silver Diamine Fluoride (SDF) is a liquid that has been proven to be an anti caries agent. Unfortunately, SDF leaves a black stain on the surface of the teeth where it was applicated, and it also has a metallic taste. Nano Silver Fluoride has the potential to replace Silver Diamine Fluoride as an anti caries agent without having the same side effects. To analyze the effect of NSF on the viability of Streptococcus mutans and Enterobacter faecalis in various phases of biofilm maturation compared to SDF, E. faecalis and S. mutans biofilm was incubated for 4 hours (adherence phase), 12 hours (active accumulation phase) and 24 hours (maturation phase) at 37 °C, then exposed to NSF at concentration (Ag
+
0,4%+F
-
2,26%), (Ag
+
0,9%+F
-
2,26%), (Ag
+
1,4%+F
-
2,26%), (Ag
+
1,9%+F
-
2,26%) for 1 hour. The percentage of viability was tested with MTT assay. Biofilm viability of E. faecalis and S. mutans in various phases showed no significant difference (p>0,05) in one-way ANOVA, compared to SDF. Results from this study showed that NSF was comparable with Silver Diamine Fluoride in inhibiting formation of E. faecalis and S. mutans biofilm.
KW - biofilm
KW - Enterobacter faecalis
KW - nano silver fluoride
KW - Streptococcus mutans
KW - viability
UR - http://www.scopus.com/inward/record.url?scp=85064849203&partnerID=8YFLogxK
U2 - 10.1063/1.5096711
DO - 10.1063/1.5096711
M3 - Conference contribution
AN - SCOPUS:85064849203
T3 - AIP Conference Proceedings
BT - 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Wulan, Praswasti P.D.K.
A2 - Gozan, Misri
A2 - Astutiningsih, Sotya
A2 - Ramahdita, Ghiska
A2 - Dhelika, Radon
A2 - Kreshanti, Prasetyanugraheni
PB - American Institute of Physics Inc.
T2 - 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
Y2 - 6 August 2018 through 8 August 2018
ER -