TY - JOUR
T1 - Evaluation of physiological alterations of the placenta through analysis of cell-free messenger ribonucleic acid concentrations of angiogenic factors
AU - Purwosunu, Yuditiya
AU - Sekizawa, Akihiko
AU - Farina, Antonio
AU - Wibowo, Noroyono
AU - Koide, Keiko
AU - Okazaki, Shiho
AU - Nakamura, Masamitsu
AU - Okai, Takashi
N1 - Funding Information:
Supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sport, and Culture of Japan (Grant 17791138) and research grants from the Japan Association of Obstetricians and Gynecologists Ogyaa Donation Foundation (T.O.) and the Takeda Science Foundation (Y.P.).
PY - 2008/1
Y1 - 2008/1
N2 - Objective: Placental messenger ribonucleic acid (mRNA) has been shown to circulate in maternal plasma. We investigated concentrations of vascular endothelial growth factor (VEGF), VEGF receptor-1 (VEGFR-1), and endoglin in subjects with preeclampsia, compared with normal pregnancies. Study Design: Peripheral blood samples were obtained from preeclampsia (n = 43) and control subjects (n = 41). Plasma ribonucleic acid was subjected to analysis by reverse transcription-polymerase chain reaction assay to examine the mRNA distribution among women with preeclampsia and control subjects during weeks 35-41 of gestation. Results: Concentrations of VEGF, VEGFR-1, and endoglin mRNA of women with preeclampsia were significantly increased. The mRNA values were observed to correlate directly with the severity of hypertension and proteinuria. VEGFR-1 mRNA was markedly elevated in women with preeclampsia and hemolysis, elevated liver enzymes, and low platelet syndrome. Conclusion: The mRNA concentrations of VEGF, VEGFR-1, and endoglin were observed to correlate directly with the severity of preeclampsia.
AB - Objective: Placental messenger ribonucleic acid (mRNA) has been shown to circulate in maternal plasma. We investigated concentrations of vascular endothelial growth factor (VEGF), VEGF receptor-1 (VEGFR-1), and endoglin in subjects with preeclampsia, compared with normal pregnancies. Study Design: Peripheral blood samples were obtained from preeclampsia (n = 43) and control subjects (n = 41). Plasma ribonucleic acid was subjected to analysis by reverse transcription-polymerase chain reaction assay to examine the mRNA distribution among women with preeclampsia and control subjects during weeks 35-41 of gestation. Results: Concentrations of VEGF, VEGFR-1, and endoglin mRNA of women with preeclampsia were significantly increased. The mRNA values were observed to correlate directly with the severity of hypertension and proteinuria. VEGFR-1 mRNA was markedly elevated in women with preeclampsia and hemolysis, elevated liver enzymes, and low platelet syndrome. Conclusion: The mRNA concentrations of VEGF, VEGFR-1, and endoglin were observed to correlate directly with the severity of preeclampsia.
KW - cell-free ribonucleic acid
KW - endoglin
KW - preeclampsia
KW - vascular endothelial growth factor
KW - vascular endothelial growth factor receptor-1
UR - http://www.scopus.com/inward/record.url?scp=37349090053&partnerID=8YFLogxK
U2 - 10.1016/j.ajog.2007.06.079
DO - 10.1016/j.ajog.2007.06.079
M3 - Article
C2 - 18166325
AN - SCOPUS:37349090053
SN - 0002-9378
VL - 198
SP - 124.e1-124.e7
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
IS - 1
ER -