@article{220cb4f7bde54a7480bd52798c44f627,
title = "Evaluation of nerve growth factor serum level for early detection of leprosy disability",
abstract = "Introduction: this research aimed to analyze nerve growth factor (NGF) contents as diagnostic tools for early disability in leprosy patients and the cut-off point value. Methods: research samples consisted of 79 leprosy patients with disability grade 0 or 1 who met the clinically approved inclusion criteria. The age of patients ranged from 14 to 50 years. For both sample groups, blood serum was collected to determine NGF concentration. NGF level was analyzed by enzyme-linked immunosorbent assay (ELISA) according to the manual guide of the kit insert from Cussabio{\textregistered}. Statistical analysis used SPSS 17 software for Windows. A comparison was performed with the Student's t-test and the NGF concentration cut-off point was determined using a receiver operating characteristic (ROC) curve. Results: the research result demonstrated that NGF concentration in multibacillary leprosy with disability grade 0 was higher than in grade 1. Leprosy with disability grade 0 had an NGF content reaching 100.46 pg/mL, while those with grade 1 had a lower concentration of NGF at 30.56 pg/mL. The higher disability grade indicated a lower NGF concentration in the blood serum. Based on the ROC analysis result, the NGF cut-off was shown to be 81.43 pg/mL. This result indicated that low NGF in nerve and skin lesions of leprosy patients contributes to early peripheral nerve malfunction due to Mycobacterium leprae infection. Conclusion: these results prove that NGF can be used as a marker of early disability in leprosy, with the cut-off value at 81.43 pg/mL.",
keywords = "Detection, Disability, Early, Growth, Leprosy, Nerve",
author = "Dhelya Widasmara and Menaldi, {Sri Linuwih} and Agus Turchan",
note = "Funding Information: Study population: the sample population consisted of 79 multibacillary (MB) type leprosy patients with disability grades 0 (n = 36) and 1 (n = 43), meeting the clinically approved inclusion criteria and selected by consecutive sampling technique. Their ages ranged from 14 to 50 years, and they were willing to participate in this research. Participants were recruited for five months at the Leprosy Kediri Hospital, East Java, Indonesia. All MB type leprosy patients who met the sample requirements and were willing to participate in the study were given an explanation of the research. Participants gave written informed consent and approval for biopsy of skin lesions. This study was approved by the Health Research Ethics Committee of the Faculty of Medicine, Airlangga University, Surabaya, with information on ethical conduct ({"}Ethical Clearance{"}) No. 341/EC/KEPK/FKUA/2014. Privacy and confidentiality were supported by principles of the Belmont Report: beneficence, justice, and respect for persons. During the informed consent process, the author informed participants about the precautions taken to protect the confidentiality of the data and the parties who may have access (e.g. research team). This allowed participants to decide about the adequacy of the protections and the acceptability of the possible release of private information to the interested parties. Publisher Copyright: {\textcopyright} 2020, African Field Epidemiology Network. All rights reserved. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2020",
month = sep,
day = "1",
doi = "10.11604/pamj.2020.37.145.15213",
language = "English",
volume = "37",
pages = "1--9",
journal = "Pan African Medical Journal",
issn = "1937-8688",
publisher = "Pan African Medical Journal",
}