TY - JOUR
T1 - Evaluation of loop-mediated isothermal amplification for detecting COVID-19
AU - Giantini, Astuti
AU - Suhaeri, Muhammad
AU - Kusumaningrum, Ardiana
AU - Prasetyo, Dimas Seto
AU - Sahar, Windy
AU - Hidayat, Rakhmad
AU - Putra, Muhammad Arza
AU - Hermawan,
N1 - Funding Information:
We thank PT Nusantara Berkat Cipta in providing this work with the COVIDNow diagnostic tool. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Publisher Copyright:
© 2022 The Author(s)
PY - 2023/2
Y1 - 2023/2
N2 - The emergence of SARS-CoV-2 has caused worldwide pandemic of COVID-19. Infection is difficult to diagnose early as some patients remain asymptomatic and may carry this virus to other people. Currently, qRT-PCR is the widely accepted mode for detection. However, the need for sophisticated instrument and trained personnel may hinder its application, especially in remote and facility-lacking areas. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) may serve as a potential approach for detection of SARS-CoV-2 as the resources needed in its application is far less complex than those of qRT-PCR. Herein, we evaluated RT-LAMP based analytical method (COVIDNow), relative to qRT-PCR, in detecting SARS-CoV-2 by using 63 clinical respiratory samples. Based on our finding, COVIDNow exhibited sensitivity and specificity values of 87.5% and 80.6%, respectively. Taken together, RT-LAMP based detection of SARS-CoV-2 by utilizing COVIDNow might serves as a valuable diagnostic tool in the management of global COVID-19 pandemic condition.
AB - The emergence of SARS-CoV-2 has caused worldwide pandemic of COVID-19. Infection is difficult to diagnose early as some patients remain asymptomatic and may carry this virus to other people. Currently, qRT-PCR is the widely accepted mode for detection. However, the need for sophisticated instrument and trained personnel may hinder its application, especially in remote and facility-lacking areas. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) may serve as a potential approach for detection of SARS-CoV-2 as the resources needed in its application is far less complex than those of qRT-PCR. Herein, we evaluated RT-LAMP based analytical method (COVIDNow), relative to qRT-PCR, in detecting SARS-CoV-2 by using 63 clinical respiratory samples. Based on our finding, COVIDNow exhibited sensitivity and specificity values of 87.5% and 80.6%, respectively. Taken together, RT-LAMP based detection of SARS-CoV-2 by utilizing COVIDNow might serves as a valuable diagnostic tool in the management of global COVID-19 pandemic condition.
KW - COVID-19
KW - Diagnosis
KW - qRT-PCR
KW - RT-LAMP
KW - SARS-CoV-2
UR - http://www.scopus.com/inward/record.url?scp=85146022198&partnerID=8YFLogxK
U2 - 10.1016/j.jcvp.2022.100132
DO - 10.1016/j.jcvp.2022.100132
M3 - Article
AN - SCOPUS:85146022198
SN - 2667-0380
VL - 3
JO - Journal of Clinical Virology Plus
JF - Journal of Clinical Virology Plus
IS - 1
M1 - 100132
ER -