Evaluation of Indonesian mangrove Xylocarpus granatum leaves ethyl acetate extract as potential anticancer drug

Jason Darmadi, Razethy Rahayu Batubara, Sandiego Himawan, Norma Nur Azizah, Hilyatushalihah Kholis Audah, Ade Arsianti, Evi Kurniawaty, Intan Safinar Ismail, Irmanida Batubara, Kholis Abdurachim Audah

Research output: Contribution to journalArticlepeer-review

Abstract

Local Xylocarpus granatum leaves were extracted by ethyl acetate solvent and characterized by TLC fingerprinting and 2D 1H NMR spectroscopy to contain phenolic compounds as well as several organic and amino acids as metabolic byproducts, such as succinic acid and acetic acid. Traces of flavonoids and other non-categorized phenolic compounds exhibited intermediate antioxidant activity (antioxidant IC50 84.93 ppm) as well as anticancer activity against HeLa, T47D, and HT-29 cell lines; which the latter being most effective against HT-29 with Fraction 5 contained the strongest activity (anticancer IC50 23.12 ppm). Extracts also behaved as a natural growth factor and nonlethal towards brine shrimps as well as human adipose-derived stem cell hADSC due to antioxidative properties. A stability test was performed to examine how storage conditions factored in bioactivity and phytochemical structure. Extracts were compared with several studies about X. granatum leaves extracts to evaluate how ethnogeography and ecosystem factored on biologically active compounds. Further research on anticancer or antioxidant mechanism on cancer cells is needed to determine whether the extract is suitable as a candidate for an anticancer drug.

Original languageEnglish
Article number6080
JournalScientific reports
Volume11
Issue number1
DOIs
Publication statusPublished - Dec 2021

Fingerprint Dive into the research topics of 'Evaluation of Indonesian mangrove Xylocarpus granatum leaves ethyl acetate extract as potential anticancer drug'. Together they form a unique fingerprint.

Cite this