@article{25f3cead52054628a244dec456c77e87,
title = "Evaluation and comparison of automated and manual ELISA for diagnosis of chronic pulmonary aspergillosis (CPA) in Indonesia",
abstract = "Pulmonary tuberculosis (TB) is one of the common risk factors for chronic pulmonary aspergillosis (CPA). A positive Aspergillus IgG is a key element of the diagnosis of CPA but this has not been studied in Indonesia. We conducted studies with patients at the end of TB therapy in Indonesia. We performed receiver operating curve (ROC) analysis to determine the optimum cutoff of the Aspergillus-specific IgG level (Immulite and Dynamiker ELISA) in those patients who met criteria of CPA in relation to control groups. In 203 TB patients, 26 (13%) patients had clinical and radiological features of CPA. We derived optimum cutoffs for Immulite Aspergillus-specific IgG of 11.5 mg/L and Dynamiker anti-galactomannan IgG of 106.8 AU/mL (sensitivity 89% and 83%, specificity 78% and 51%, respectively). The currently accepted Aspergillus-specific IgG cutoff of Immulite and Dynamiker assays for CPA diagnosis may require slight adjustment for the Indonesian population.",
keywords = "Aspergillosis, Immunoglobulin G, Indonesia",
author = "Anna Rozaliyani and Ridhawati Syam and Robiatul Adawiyah and Mulyati Tugiran and Sari, {Cut Yulia I.} and Erlina Burhan and Retno Wahyuningsih and Riina Rautemaa-Richardson and Denning, {David W.} and FINDRA SETIANINGRUM",
note = "Funding Information: We are indebted to Dr. Diah Handayani from Persahabatan Hospital, Dr. Johannes Ramnath from Universitas Kristen Indonesia Hospital, Dr. Arief Riadi Arifin from MH Thamrin Hospital, Dr. Martin Rumende from Cipto Mangunkusumo Hospital, and Dr. Ammar Hasyim for identifying eligible patients and assisting in patient's recruitment. We gratefully acknowledge Dr. Finny Nandipinto for providing radiological expertise of the patients. We are grateful to all the staffs at the Department of Parasitology Faculty of Medicine University of Indonesia for their help in sample storage and testing of sputum samples. We thank Dr. John Belcher of the department of medical statistics for statistical support. We would like to thank the staffs at the Mycology Reference Centre Manchester for providing training of manual Aspergillus-specific IgG ELISA tests and support throughout the project. We would like to thank Siemens Indonesia and Prodia Laboratory for their assistance in performing Immulite Aspergillus-specific IgG tests. We would like to thank Dynamiker for kindly donating test kits to perform this study. The scholarship to support the studies of the first author was provided by Lembaga Pengelola Dana Pendidikan (LPDP), Republik Indonesia. Prof D Denning and Dr. Rautemaa-Richardson are partly supported by the NIHR Manchester Biomedical Research Centre. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = nov,
doi = "10.1016/j.diagmicrobio.2020.115124",
language = "English",
volume = "98",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier Inc.",
number = "3",
}
