Evaluation and comparison of automated and manual ELISA for diagnosis of chronic pulmonary aspergillosis (CPA) in Indonesia

Findra Setianingrum, Anna Rozaliyani, Ridhawati Syam, Robiatul Adawiyah, Mulyati Tugiran, Cut Yulia I. Sari, Erlina Burhan, Retno Wahyuningsih, Riina Rautemaa-Richardson, David W. Denning

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Pulmonary tuberculosis (TB) is one of the common risk factors for chronic pulmonary aspergillosis (CPA). A positive Aspergillus IgG is a key element of the diagnosis of CPA but this has not been studied in Indonesia. We conducted studies with patients at the end of TB therapy in Indonesia. We performed receiver operating curve (ROC) analysis to determine the optimum cutoff of the Aspergillus-specific IgG level (Immulite and Dynamiker ELISA) in those patients who met criteria of CPA in relation to control groups. In 203 TB patients, 26 (13%) patients had clinical and radiological features of CPA. We derived optimum cutoffs for Immulite Aspergillus-specific IgG of 11.5 mg/L and Dynamiker anti-galactomannan IgG of 106.8 AU/mL (sensitivity 89% and 83%, specificity 78% and 51%, respectively). The currently accepted Aspergillus-specific IgG cutoff of Immulite and Dynamiker assays for CPA diagnosis may require slight adjustment for the Indonesian population.

Original languageEnglish
Article number115124
JournalDiagnostic Microbiology and Infectious Disease
Volume98
Issue number3
DOIs
Publication statusPublished - Nov 2020

Keywords

  • Aspergillosis
  • Immunoglobulin G
  • Indonesia

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