Ethyl acetate fraction of garcinia mangostana-linn pericarp extract: Anti - Candida albicans and epithelial cytotoxicity

Candida species are a commensal agent in oral, skin, and gastrointestinal tract environment, but when there is an underlying predisposing condition in the host, it causing candida infections. The increasing of antifungal resistance complicates patient management. Therefore, it is necessary to identify potential antifungal agents. Garcinia mangostana‑Linn (GML) is a famous tropical fruit in Indonesia and other southeast Asia countries. This study was conducted to examine the antifungal activity of chromatographic column ethyl acetate fraction of GML pericarp against Candida albicans. GML was extracted with ethanol and fractionated with ethyl acetate. C. albicans ATCC 10231 was used in this study. Antifungal activities were expressed as the viability of C. albicans growth identification with 3‑4,5‑Dimethylthiazol‑2‑yl)‑2,5‑dipenyltetrazolium bromide assay. There is no previous research that analyzes the antifungal effect of GML based on ethyl acetate fraction from the chromatographic fractionation technique, especially as anti‑C. albicans. In this study, we also conducted an analysis of the cytotoxicity effect of ethyl acetate fraction on HaCaT cell line. The result of our study indicates that ethyl acetate fraction of GML pericarp extract has reduced the viability of C. albicans. There is a tendency of decrease in viability in line with the increase of the concentration of fraction. As anti‑C. albicans ethyl acetate fraction of GML pericarp extract has no cytotoxicity to HaCaT cell line. This result showed the ability of ethyl acetate fraction of GML extract changes the viability of C. albicans, but not toxic to HaCaT cell line. It may consider ethyl acetate fraction of GML pericarp extract as potent anti‑C. albicans and promising adjuncts in oral health product.