TY - JOUR
T1 - Ethanol Extract of Detam 1 Soybean Seed (Glycine Max L. Merr) for Chronic Kidney Disease Therapy by In Vitro Study
AU - Prahastuti, Sijani
AU - Hidayat, Meilinah
AU - Hasiana, Stella Tinia
AU - Widowati, Wahyu
AU - Amalia, Annisa
AU - Qodariah, Rismawati Laila
AU - Rizal, Rizal
AU - Wibowo, Satrio Haryo Benowo
AU - Kusuma, Hanna Sari Widya
N1 - Funding Information:
We gratefully acknowledge the financial support of Penelitian Terapan Unggulan Perguruan Tinggi 2018 from Directorate General of Higher Education, Ministry of Research, Technology and Higher Education of the Republic of Indonesia. This study supported by Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia. Moreover, we are thankful to Dewani Tediana Yusepany, Dwi Surya Artie, Kamila Yashfa Gunawan, Enden Dea Nataya, Ika Adhani Sholihah, Rr. Anisa Siwi Handayani, Alya Mardhotillah Azizah, Riyani Lestari from Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung for their valuable assistance.
Publisher Copyright:
© 2019, Universitas Gadjah Mada - Faculty of Pharmacy. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Chronic Kidney Disease (CKD) has increased incidence and prevalence in developing nations. In this in vitro study, we evaluated the cells proliferative effects, fibronectin (FN), transforming growth factor β (TGF-β1), and Reactive oxygen species (ROS)-level inhibition potential of ethanol extract of detam 1 soybean seed (EEDS) on glucose-induced kidney mesangial cells (SV40 MES 13). The cells proliferation assay used 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)2H-tetrazolium (MTS) assay. FN and TGF-β1 level were measured using ELISA assay kit and ROS level using flow cytometry. Level of FN, TGF-β1 and ROS, on CKD cells model (5 mM, 10 mM glucose-induced mesangial cell) treated with EEDS 6.25 µg/mL were lower significantly compared to positive control, EEDS improve cells viability and decrease FN, TGF-β1 and ROS level in glucose-induced kidney mesangial cells as CKD cells model.
AB - Chronic Kidney Disease (CKD) has increased incidence and prevalence in developing nations. In this in vitro study, we evaluated the cells proliferative effects, fibronectin (FN), transforming growth factor β (TGF-β1), and Reactive oxygen species (ROS)-level inhibition potential of ethanol extract of detam 1 soybean seed (EEDS) on glucose-induced kidney mesangial cells (SV40 MES 13). The cells proliferation assay used 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)2H-tetrazolium (MTS) assay. FN and TGF-β1 level were measured using ELISA assay kit and ROS level using flow cytometry. Level of FN, TGF-β1 and ROS, on CKD cells model (5 mM, 10 mM glucose-induced mesangial cell) treated with EEDS 6.25 µg/mL were lower significantly compared to positive control, EEDS improve cells viability and decrease FN, TGF-β1 and ROS level in glucose-induced kidney mesangial cells as CKD cells model.
KW - chronic kidney disease
KW - fibronectin
KW - ROS
KW - soybean
KW - TGF-β1
UR - http://www.scopus.com/inward/record.url?scp=85151696155&partnerID=8YFLogxK
U2 - 10.22146/mot.45053
DO - 10.22146/mot.45053
M3 - Article
AN - SCOPUS:85151696155
SN - 1410-5918
VL - 24
SP - 160
EP - 168
JO - Majalah Obat Tradisional
JF - Majalah Obat Tradisional
IS - 3
ER -