Establishment of primary 3D cell culture based on magnetic bioprinting for colorectal cancer cells from patients in Cipto Mangunkusumo National Hospital Indonesia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Colorectal cancer is the third most common malignancy with more than 1.2 million new annual cases in the world. Methods for propagating cancer cells range from conventional to the more advanced system. Cancer cells are routinely cultured in two-dimensional (2D) model. This method faces several limitations attributable to lack of structural architecture and nature condition. Three-dimensional (3D) model mimics in vivo environment more closely than the conventional 2D. This strategy may help in elucidating cancer biology and subsequently contribute to higher success of therapy. The goal of this study was to establish the primary 3D culture from colorectal cancer collected from patients in Cipto Mangunkusumo National Hospital. Cancer cells were extracted mechanically and grown in DMEM/F12 medium supplemented with serum and antibiotics. The NanoShuttle-PL was added to the cells and magnetic drive was used to form cell spheroids. There were 22 samples obtained with varying cancer stages viz. IIA, IIIB, IIIC, and IV. Primary culture of cancer cells successfully established by mechanically extracting the tumour tissues into small pieces. Results on magnetic bioprinting revealed that the 3D culture (spheroids) was successfully established rapidly. Further studies are needed to evaluate culture viability and cell compositions, as well as performing the chemosensitivity testing.

Original languageEnglish
Title of host publicationProceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019
Subtitle of host publicationTowards Innovative Research and Cross-Disciplinary Collaborations
EditorsNaji Arafat Mahat, Roswanira Ab. Wahab, Fahrul Zaman Huyop, Aemi Syazwani Abdul Keyon, Nursyafreena Binti Attan, Sheela Chandren, Ida Bagus Wayan Gunam
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419001
DOIs
Publication statusPublished - 6 Sep 2019
Event2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019 - Bali, Indonesia
Duration: 11 Apr 201912 Apr 2019

Publication series

NameAIP Conference Proceedings
Volume2155
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019
CountryIndonesia
CityBali
Period11/04/1912/04/19

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  • Cite this

    Abdullah, M., Bela, B., Syam, A. F., Simadibrata, M., Meilany, S., Annisa, F., Amirulloh, D., Makmun, D., & Rani, A. A. (2019). Establishment of primary 3D cell culture based on magnetic bioprinting for colorectal cancer cells from patients in Cipto Mangunkusumo National Hospital Indonesia. In N. A. Mahat, R. A. Wahab, F. Z. Huyop, A. S. A. Keyon, N. B. Attan, S. Chandren, & I. B. W. Gunam (Eds.), Proceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019: Towards Innovative Research and Cross-Disciplinary Collaborations [020025] (AIP Conference Proceedings; Vol. 2155). American Institute of Physics Inc.. https://doi.org/10.1063/1.5125529