TY - GEN
T1 - Establishment of primary 3D cell culture based on magnetic bioprinting for colorectal cancer cells from patients in Cipto Mangunkusumo National Hospital Indonesia
AU - Abdullah, Murdani
AU - Bela, Budiman
AU - Syam, Ari Fahrial
AU - Simadibrata, Marcellus
AU - Meilany, Sofy
AU - Annisa, Firda
AU - Amirulloh, Dian
AU - Makmun, Dadang
AU - Rani, Abdul Aziz
N1 - Funding Information:
The authors are thankful to Ministry of Research technology and higher education of Republic of Indonesia for providing funding for conducting this research project.
Publisher Copyright:
© 2019 Author(s).
PY - 2019/9/6
Y1 - 2019/9/6
N2 - Colorectal cancer is the third most common malignancy with more than 1.2 million new annual cases in the world. Methods for propagating cancer cells range from conventional to the more advanced system. Cancer cells are routinely cultured in two-dimensional (2D) model. This method faces several limitations attributable to lack of structural architecture and nature condition. Three-dimensional (3D) model mimics in vivo environment more closely than the conventional 2D. This strategy may help in elucidating cancer biology and subsequently contribute to higher success of therapy. The goal of this study was to establish the primary 3D culture from colorectal cancer collected from patients in Cipto Mangunkusumo National Hospital. Cancer cells were extracted mechanically and grown in DMEM/F12 medium supplemented with serum and antibiotics. The NanoShuttle-PL was added to the cells and magnetic drive was used to form cell spheroids. There were 22 samples obtained with varying cancer stages viz. IIA, IIIB, IIIC, and IV. Primary culture of cancer cells successfully established by mechanically extracting the tumour tissues into small pieces. Results on magnetic bioprinting revealed that the 3D culture (spheroids) was successfully established rapidly. Further studies are needed to evaluate culture viability and cell compositions, as well as performing the chemosensitivity testing.
AB - Colorectal cancer is the third most common malignancy with more than 1.2 million new annual cases in the world. Methods for propagating cancer cells range from conventional to the more advanced system. Cancer cells are routinely cultured in two-dimensional (2D) model. This method faces several limitations attributable to lack of structural architecture and nature condition. Three-dimensional (3D) model mimics in vivo environment more closely than the conventional 2D. This strategy may help in elucidating cancer biology and subsequently contribute to higher success of therapy. The goal of this study was to establish the primary 3D culture from colorectal cancer collected from patients in Cipto Mangunkusumo National Hospital. Cancer cells were extracted mechanically and grown in DMEM/F12 medium supplemented with serum and antibiotics. The NanoShuttle-PL was added to the cells and magnetic drive was used to form cell spheroids. There were 22 samples obtained with varying cancer stages viz. IIA, IIIB, IIIC, and IV. Primary culture of cancer cells successfully established by mechanically extracting the tumour tissues into small pieces. Results on magnetic bioprinting revealed that the 3D culture (spheroids) was successfully established rapidly. Further studies are needed to evaluate culture viability and cell compositions, as well as performing the chemosensitivity testing.
UR - http://www.scopus.com/inward/record.url?scp=85072617439&partnerID=8YFLogxK
U2 - 10.1063/1.5125529
DO - 10.1063/1.5125529
M3 - Conference contribution
AN - SCOPUS:85072617439
T3 - AIP Conference Proceedings
BT - Proceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019
A2 - Mahat, Naji Arafat
A2 - Wahab, Roswanira Ab.
A2 - Huyop, Fahrul Zaman
A2 - Keyon, Aemi Syazwani Abdul
A2 - Attan, Nursyafreena Binti
A2 - Chandren, Sheela
A2 - Gunam, Ida Bagus Wayan
PB - American Institute of Physics Inc.
T2 - 2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019
Y2 - 11 April 2019 through 12 April 2019
ER -
