TY - JOUR
T1 - Eradication effect of javanese turmeric (Curcuma xanthorrhiza. roxb.) extract on the early phase of candida albicans biofilm
AU - Puspitawati, Ria
AU - Lewiyonah, Rista
AU - Herdiantoputri, Ranny Rahaningrum
AU - Gultom, Ferry P
AU - Sastradipura, Dewi Fatma Suniarti
N1 - Publisher Copyright:
© 2017 The Authors. Published by Innovare Academic Sciences Pvt Ltd.
PY - 2017/12
Y1 - 2017/12
N2 - Objectives: To analyze the effect of Javanese turmeric extract on eradicating the initial phase of C. albicans biofilm formation. Methods: A 100 µl of C. albicans suspension 10−4 was cultured on a 96 - well plate, incubated for 1.5 and 3 h at 37° C and then aspirated and washed by phosphate buffer saline before exposure to 100 µl of 5%, 10%, 15%, 20% 25%, 30%, and 35% Javanese turmeric extract for 24 h. The positive control was 100 µl of nystatin 100,000 IU. Results: The eradication effect of 35% Javanese turmeric extract at the two stages of initial phase biofilm was comparable with those of nystatin. The viability of C. albicans exposed to the extract at an advanced stage of the early phase of biofilm formation tended to be higher than that at the early stage, but the difference was not significant. Conclusion: The in vitro antifungal effect of Javanese turmeric extract against C. albicans could be effective in eradicating yeast in the early phase of biofilm formation.
AB - Objectives: To analyze the effect of Javanese turmeric extract on eradicating the initial phase of C. albicans biofilm formation. Methods: A 100 µl of C. albicans suspension 10−4 was cultured on a 96 - well plate, incubated for 1.5 and 3 h at 37° C and then aspirated and washed by phosphate buffer saline before exposure to 100 µl of 5%, 10%, 15%, 20% 25%, 30%, and 35% Javanese turmeric extract for 24 h. The positive control was 100 µl of nystatin 100,000 IU. Results: The eradication effect of 35% Javanese turmeric extract at the two stages of initial phase biofilm was comparable with those of nystatin. The viability of C. albicans exposed to the extract at an advanced stage of the early phase of biofilm formation tended to be higher than that at the early stage, but the difference was not significant. Conclusion: The in vitro antifungal effect of Javanese turmeric extract against C. albicans could be effective in eradicating yeast in the early phase of biofilm formation.
KW - Candida albicans biofilm
KW - Curcuma xanthorrhiza Roxb
KW - Javanese turmeric extract
KW - Viability
UR - http://www.scopus.com/inward/record.url?scp=85042023754&partnerID=8YFLogxK
U2 - 10.22159/ijap.2017.v9s2.29
DO - 10.22159/ijap.2017.v9s2.29
M3 - Article
AN - SCOPUS:85042023754
SN - 0975-7058
VL - 9
SP - 117
EP - 120
JO - International Journal of Applied Pharmaceutics
JF - International Journal of Applied Pharmaceutics
IS - Special Issue 2
ER -