TY - JOUR
T1 - (+)-EPOXYDONE
T2 - A MAJOR SECONDARY METABOLITE AS ANTIBACTERIAL AGENT FROM Phomopsis sp. TcBt1Bo-6 ISOLATED FROM STEM OF BROTOWALI PLANT (Tinospora crispa)
AU - Fathoni, A.
AU - Hudiyono, S.
AU - Cahyana, A. H.
AU - Nurkanto, A.
AU - Agusta, A.
N1 - Funding Information:
All authors would like to express thanks to Saintek-BRIN Scholarship for giving the Doctoral scholarship to the first author and Kemendikbudristek for the PDD research grant 2022 for funding this research and publication. The authors also thank the scientific and technical support, and also facilities of Advanced Characterization Lab., Research Center for Chemistry, Serpong, through e-Layanan Sains (Elsa), National Research and Innovation Agency (BRIN). All authors equally contributed to this work as main contributors.
Publisher Copyright:
© 2022, Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma. All rights reserved.
PY - 2022/10/1
Y1 - 2022/10/1
N2 - Isolation, characterization, and bioproduction capacity of (+)-epoxydone produced by Phomopsis sp. TcBt1Bo-6 was performed in this present report. The fungus was cultured in potatoes dextrose broth (PDB) medium. Isolation and purification of (+)-epoxydone using chromatography methods. The metabolite structure elucidation was carried out by using UV, FTIR, 1D/2D-NMR, MS, and optical rotation, and compared with other reports. This fungus has the bioproduction ability of (+)-epoxydone of 189.43 mg/L. The metabolite of (+)-epoxydone has potent as antibacterial and antimycobacterial against E.coli, S.aureus, and M.smegmatis with MIC values of 25, > 50, and 50 (partial) μg/mL, respectively. This study is the new report on the bioproduction of bioactive metabolite (+)-epoxydone as well as the antibacterial and antimycobacterial activities of this compound.
AB - Isolation, characterization, and bioproduction capacity of (+)-epoxydone produced by Phomopsis sp. TcBt1Bo-6 was performed in this present report. The fungus was cultured in potatoes dextrose broth (PDB) medium. Isolation and purification of (+)-epoxydone using chromatography methods. The metabolite structure elucidation was carried out by using UV, FTIR, 1D/2D-NMR, MS, and optical rotation, and compared with other reports. This fungus has the bioproduction ability of (+)-epoxydone of 189.43 mg/L. The metabolite of (+)-epoxydone has potent as antibacterial and antimycobacterial against E.coli, S.aureus, and M.smegmatis with MIC values of 25, > 50, and 50 (partial) μg/mL, respectively. This study is the new report on the bioproduction of bioactive metabolite (+)-epoxydone as well as the antibacterial and antimycobacterial activities of this compound.
KW - (+)-Epoxydone
KW - Bioproduction
KW - Characterization
KW - Isolation
KW - Phomopsis sp. TcBt1Bo-6
UR - http://www.scopus.com/inward/record.url?scp=85145395515&partnerID=8YFLogxK
U2 - 10.31788/RJC.2022.1546982
DO - 10.31788/RJC.2022.1546982
M3 - Article
AN - SCOPUS:85145395515
SN - 0974-1496
VL - 15
SP - 2210
EP - 2217
JO - Rasayan Journal of Chemistry
JF - Rasayan Journal of Chemistry
IS - 4
ER -