Enzymatic synthesis of glycerol and sucrose-palm oil fatty acid esters produced and their potency as antimicrobial agents

R. A. Naulidia, E. Juliana, S. Handayani, F. Damayanti, S. Setiasih, S. Hudiyono

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Enzymatic synthesis of glycerol-palm oil fatty acid and sucrose-palm oil fatty acid esters was conducted using Candida rugosa lipase EC 3.1.1.3 in n-hexane. The palm oil fatty acid was obtained by hydrolyzing palm oil using KOH in 96% ethanol. The molar ratio of fatty acid to glycerol were varied, i.e. 1:1, 1:2, 1:3 and 1:4, while fatty acid to sucrose were 1:0.2, 1:0.4, 1:0.6 and 1:0.8. Conversion percentage of esterification was determined by titrimetric method. The highest conversion percentage was obtained at mole ratio 1:4 with the value of 42% for glycerol-palm oil fatty acid ester and at mole ratio 1:0.8 with the value of 24% for sucrose-palm oil fatty acid ester. Characterization esterification products by using FT-IR showed that the ester bond was formed as indicated at 1748 cm-1 for the absorption peak of C=O ester group of glycerol-fatty acid ester and 1734 cm-1 for sucrose-fatty acid ester. Both esters were then examined by antimicrobial assay. This study showed that glycerol-palm oil fatty acid ester can not inhibit the growth of Staphylococcus epidermidis and Propionibacterium acnes, while sucrose-fatty acid ester have ability to inhibit the growth of Staphylococcus aureus but not for Escherichia coli.

Original languageEnglish
Title of host publication8th International Conference of the Indonesian Chemical Society, ICICS 2019
EditorsMohamad Rafi, Rudi Heryanto, Wulan Tri Wahyuni
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735420021
DOIs
Publication statusPublished - 4 Jun 2020
Event8th International Conference of the Indonesian Chemical Society 2019, ICICS 2019 - Bogor, Indonesia
Duration: 6 Aug 20197 Aug 2019

Publication series

NameAIP Conference Proceedings
Volume2243
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference8th International Conference of the Indonesian Chemical Society 2019, ICICS 2019
CountryIndonesia
CityBogor
Period6/08/197/08/19

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    Naulidia, R. A., Juliana, E., Handayani, S., Damayanti, F., Setiasih, S., & Hudiyono, S. (2020). Enzymatic synthesis of glycerol and sucrose-palm oil fatty acid esters produced and their potency as antimicrobial agents. In M. Rafi, R. Heryanto, & W. T. Wahyuni (Eds.), 8th International Conference of the Indonesian Chemical Society, ICICS 2019 [020013] (AIP Conference Proceedings; Vol. 2243). American Institute of Physics Inc.. https://doi.org/10.1063/5.0001346