Abstract
Aim of this study was to synthesisfatty acid ethyl ester compound of hydrolyzed castor oil and its oxidation product using Candida rugosalipase. Both of the esterification products were expected to have antimicrobial activity against Staphylococcus epidermidisand Propionibacterium acnesand may act as emulsifiers. Optimization of esterification reactions was done by varying the mole ratio between fatty acids andethanol, ie 1:1, 1:2, 1:3, and 1:4. The esterification products were then characterized using FTIR. Conversion percentage was determined by titrimetric method, to calculate the amount of fatty acids that wasconverted to ester. The ester productwas tested its ability as emulsifierby emulsifier test. The esterification products were also examined their antimicrobial activity using disc diffusion method. The highest conversion percentage for fatty acid ethyl ester and its oxidation product were 76 % and72% respectively. Characterization using FTIR for both ester showed that the absorption band of C=O ester functional group at wave number 1731.27 cm-1and 1732.15 cm-1respectively. The emulsifier test showed that both esters have ability to stabilize emulsion up to 24 hours for water-in-oil emulsion (w/o) type. Antimicrobial assay showed that both esters have antimicrobial activity against both bacteria
| Original language | English |
|---|---|
| Title of host publication | Proceedings of BROMO Conference (BROMO 2018) - Symposium on Natural Product and Biodiversity |
| Publisher | SciTePress |
| Pages | 1-6 |
| ISBN (Print) | 978-989-758-347-6 |
| Publication status | Published - 2018 |