Enrichment of the gamma oryzanol level from rice bran by addition of inorganic salts on ionic liquid 1-butyl-3- methylimidazolium hexafluorophosphate ([BMIM] PF₆) extraction

Objective: Rice bran oil has many helath benefit. The biologically active compounds of rice bran oil are fatty acids, squalene, tocopherols, phytosterols, tocotrienols, oryzanol, and polyphenols. Gamma oryzanol is a combination of at least 10 components of ferulic acid esters and alcohols triterpene. Gamma oryzanol has pharmacological activity includes cardiovascular disease, antioxidant, anticancer, antidiabetic, antiulcerogenic, neuroprotective and action immunomodulator. Ionic liquids are an environmentally friendly solvent used in the extraction and separation of bioactive compounds from plants. This study was conducted to evaluate the effect of inorganic salts addition on ionic liquid [Bmim]PF₆ in increasing levels of gamma oryzanol from rice bran. Method: In the experiments, IL [Bmim]PF₆ - MAE method was used to separated gamma oryzanol compound, and then was partitioned with hexane after the addition of inorganic salts: KH₂PO₄, NaCl, NaNO₃ and Na₂CO₃, respectively. Gamma oryzanol content was determined by HPLC analysis. A mixture of mobile phase (methanol:acetonitrile:isopropan ol (5:4:1) and isocratic conditions at a wavelength of 327 nm. The flow rate is set at 1 ml / min. Results: The highest levels of γ-oryzanol was produced from the addition in KH₂PO₄ +: [Bmim] PF₆ by 0.26 ± 0.001 mg/g. The mechanism of salting-out effect of the compound due to the interaction of different types of interactions between solutes (ions of inorganic salts and ionic liquid) and solvent. Conclusion: The addition of inorganic salt increased the gamma oryzanol level. The addition of KH₂PO₄ on IL-MAE gave the highest level of gamma oryzanol.