TY - GEN
T1 - Enhancement of phycocyanin extraction from Spirulina platensis by freeze-thaw method
T2 - 17th International Conference on Quality in Research, QiR 2021 in conjunction with the International Tropical Renewable Energy Conference 2021, I-Trec 2021 and the 2nd AUN-SCUD International Conference, CAIC-SIUD
AU - Sianipar, Andri Josua
AU - Dianursanti, Dianursanti
N1 - Publisher Copyright:
© 2024 Author(s).
PY - 2024/2/6
Y1 - 2024/2/6
N2 - Spirulina platensis is a cyanobacterial species that is rich in high-value biochemicals, including phycocyanin at a significant level. Phycocyanin is a light blue phycobiliprotein pigment. Phycocyanin can be used as a food coloring, for biochemical analysis, and as an active ingredient in pharmaceutical and nutraceutical products. To obtain phycocyanin in sufficient quantity and purity, an effective and efficient extraction technique is needed. Freeze-thaw is one of the well-known method for the phycocyanin and the synergy with ultrasonication method showed a promising enhancement. This study aimed to evaluate the phycocyanin extraction effectiveness by the synergy of freeze-thaw with the ultrasonication method. Beside that, this study also optimized the duration of freezing procedure. The extraction performance was evaluated based on the phycocyanin yield (YPC) and purity level (PPC) measurements. The result showed that ultrasonication gave crude extract with higher YPC rather than the crude extract from the freeze-thaw process alone. Meanwhile, the 3 hours of freezing was the most optimal freezing duration with YPC = 36.376 mg/g and PPC = 0.827.
AB - Spirulina platensis is a cyanobacterial species that is rich in high-value biochemicals, including phycocyanin at a significant level. Phycocyanin is a light blue phycobiliprotein pigment. Phycocyanin can be used as a food coloring, for biochemical analysis, and as an active ingredient in pharmaceutical and nutraceutical products. To obtain phycocyanin in sufficient quantity and purity, an effective and efficient extraction technique is needed. Freeze-thaw is one of the well-known method for the phycocyanin and the synergy with ultrasonication method showed a promising enhancement. This study aimed to evaluate the phycocyanin extraction effectiveness by the synergy of freeze-thaw with the ultrasonication method. Beside that, this study also optimized the duration of freezing procedure. The extraction performance was evaluated based on the phycocyanin yield (YPC) and purity level (PPC) measurements. The result showed that ultrasonication gave crude extract with higher YPC rather than the crude extract from the freeze-thaw process alone. Meanwhile, the 3 hours of freezing was the most optimal freezing duration with YPC = 36.376 mg/g and PPC = 0.827.
UR - http://www.scopus.com/inward/record.url?scp=85185804787&partnerID=8YFLogxK
U2 - 10.1063/5.0157713
DO - 10.1063/5.0157713
M3 - Conference contribution
AN - SCOPUS:85185804787
T3 - AIP Conference Proceedings
BT - AIP Conference Proceedings
A2 - Kusuma, Andyka
A2 - Fatriansyah, Jaka Fajar
A2 - Dhelika, Radon
A2 - Pratama, Mochamad Adhiraga
A2 - Irwansyah, Ridho
A2 - Maknun, Imam Jauhari
A2 - Putra, Wahyuaji Narottama
A2 - Ardi, Romadhani
A2 - Harwahyu, Ruki
A2 - Harahap, Yulia Nurliani
A2 - Lischer, Kenny
PB - American Institute of Physics Inc.
Y2 - 13 October 2021 through 15 October 2021
ER -
