Enhanced Growth and Lipid Production of Indonesia Indigenous Filamentous Cyanobacteria (Mastigocladus HS-46) in a Flat Photobioreactor Using Fertilizer Medium

Research on biomass and lipid production from Mastigocladus HS-46, an Indonesian indigenous filamentous microalga from Maribaya Hotspring as potential biofuel raw material has been done. This study was done to determine the effect of bean sprout extract various concentrations in 350 ppm NPK media on the biomass weight and lipid percentage of Mastigocladus HS-46 cultivated in Flat Photobioreactor (FPBR). Variations of growth media in this study are 350 ppm NPK Fertilizer medium with the addition of 1%, 2%, and 3% bean sprout extract concentrations. Besides, BBM (Bold’s Basal Media) was used as a control media. The results showed that Mastigocladus HS-46 produced the highest biomass (0.1632 g/mL) and lipid percentage (62%) when cultivated in 350 ppm NPK media with 3% bean sprout extract. Nevertheless, The Kruskal-Wallis test (α=0.05) result showed there is no significant difference in the biomass average weight of this strain, given the addition of various concentrations of bean sprout extract in NPK Fertilizer medium. Therefore, Flat photobioreactor (FPBR) as a growth container and NPK Fertilizer media with addition of bean sprout extract as a growth media are considered suitable to grow Mastigocladus HS-46 as a future biofuel raw material.