Enhanced bone regeneration capability of chitosan sponge coated with TiO2 nanoparticles

Radyum Ikono; Ni Li; Nanda Hendra Pratama; Agnia Vibriani; Diah Retno Yuniarni; Muhammad Luthfansyah; Boy Muchlis Bachtiar; Endang Winiati Bachtiar; Kamarza Mulia; Mohammad Nasikin; Hideaki Kagami; Xianqi Li; Etik Mardliyati; Nurul Taufiqu Rochman; Tokiko Nagamura-Inoue; Arinobu Tojo

doi:10.1016/j.btre.2019.e00350

Enhanced bone regeneration capability of chitosan sponge coated with TiO₂ nanoparticles

Radyum Ikono, Ni Li, Nanda Hendra Pratama, Agnia Vibriani, Diah Retno Yuniarni, Muhammad Luthfansyah, Boy Muchlis Bachtiar, Endang Winiati Bachtiar, Kamarza Mulia, Mohammad Nasikin, Hideaki Kagami, Xianqi Li, Etik Mardliyati, Nurul Taufiqu Rochman, Tokiko Nagamura-Inoue, Arinobu Tojo

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

Chitosan has been a popular option for tissue engineering, however exhibits limited function for bone regeneration due to its low mechanical robustness and non-osteogenic inductivity. Here we hybridized chitosan with TiO₂ nanoparticles to improve its bone regeneration capability. Morphology and crystallographic analysis showed that TiO₂ nanoparticles in anatase-type were distributed evenly on the surface of the chitosan sponges. Degradation test showed a significant effect of TiO₂ nanoparticles addition in retaining its integrity. Biomineralization assay using simulated body fluid showed apatite formation in sponges surface as denoted by PO⁴⁻ band observed in FTIR results. qPCR analysis supported chitosan - TiO₂ sponges in bone regeneration capability as indicated by DMP1 and OCN gene upregulation in TiO₂ treated group. Finally, cytotoxicity analysis supported the fact that TiO₂ nanoparticles added sponges were proved to be biocompatible. Results suggest that chitosan-50% TiO₂ nanoparticles sponges could be a potential novel scaffold for bone tissue engineering.

Original language	English
Article number	e00350
Journal	Biotechnology Reports
Volume	24
DOIs	https://doi.org/10.1016/j.btre.2019.e00350
Publication status	Published - 1 Dec 2019

Keywords

Bone regeneration
Chitosan
Sponges
TiO nanoparticles
Tissue engineering

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Ikono, R., Li, N., Pratama, N. H., Vibriani, A., Yuniarni, D. R., Luthfansyah, M., Bachtiar, B. M., Bachtiar, E. W., Mulia, K., Nasikin, M., Kagami, H., Li, X., Mardliyati, E., Rochman, N. T., Nagamura-Inoue, T., & Tojo, A. (2019). Enhanced bone regeneration capability of chitosan sponge coated with TiO₂ nanoparticles. Biotechnology Reports, 24, Article e00350. https://doi.org/10.1016/j.btre.2019.e00350

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abstract = "Chitosan has been a popular option for tissue engineering, however exhibits limited function for bone regeneration due to its low mechanical robustness and non-osteogenic inductivity. Here we hybridized chitosan with TiO2 nanoparticles to improve its bone regeneration capability. Morphology and crystallographic analysis showed that TiO2 nanoparticles in anatase-type were distributed evenly on the surface of the chitosan sponges. Degradation test showed a significant effect of TiO2 nanoparticles addition in retaining its integrity. Biomineralization assay using simulated body fluid showed apatite formation in sponges surface as denoted by PO4− band observed in FTIR results. qPCR analysis supported chitosan - TiO2 sponges in bone regeneration capability as indicated by DMP1 and OCN gene upregulation in TiO2 treated group. Finally, cytotoxicity analysis supported the fact that TiO2 nanoparticles added sponges were proved to be biocompatible. Results suggest that chitosan-50% TiO2 nanoparticles sponges could be a potential novel scaffold for bone tissue engineering.",

keywords = "Bone regeneration, Chitosan, Sponges, TiO nanoparticles, Tissue engineering",

author = "Radyum Ikono and Ni Li and Pratama, {Nanda Hendra} and Agnia Vibriani and Yuniarni, {Diah Retno} and Muhammad Luthfansyah and Bachtiar, {Boy Muchlis} and Bachtiar, {Endang Winiati} and Kamarza Mulia and Mohammad Nasikin and Hideaki Kagami and Xianqi Li and Etik Mardliyati and Rochman, {Nurul Taufiqu} and Tokiko Nagamura-Inoue and Arinobu Tojo",

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doi = "10.1016/j.btre.2019.e00350",

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Ikono, R, Li, N, Pratama, NH, Vibriani, A, Yuniarni, DR, Luthfansyah, M, Bachtiar, BM , Bachtiar, EW , Mulia, K , Nasikin, M, Kagami, H, Li, X, Mardliyati, E, Rochman, NT, Nagamura-Inoue, T & Tojo, A 2019, 'Enhanced bone regeneration capability of chitosan sponge coated with TiO₂ nanoparticles', Biotechnology Reports, vol. 24, e00350. https://doi.org/10.1016/j.btre.2019.e00350

TY - JOUR

T1 - Enhanced bone regeneration capability of chitosan sponge coated with TiO2 nanoparticles

AU - Ikono, Radyum

AU - Li, Ni

AU - Pratama, Nanda Hendra

AU - Vibriani, Agnia

AU - Yuniarni, Diah Retno

AU - Luthfansyah, Muhammad

AU - Bachtiar, Boy Muchlis

AU - Bachtiar, Endang Winiati

AU - Mulia, Kamarza

AU - Nasikin, Mohammad

AU - Kagami, Hideaki

AU - Li, Xianqi

AU - Mardliyati, Etik

AU - Rochman, Nurul Taufiqu

AU - Nagamura-Inoue, Tokiko

AU - Tojo, Arinobu

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Chitosan has been a popular option for tissue engineering, however exhibits limited function for bone regeneration due to its low mechanical robustness and non-osteogenic inductivity. Here we hybridized chitosan with TiO2 nanoparticles to improve its bone regeneration capability. Morphology and crystallographic analysis showed that TiO2 nanoparticles in anatase-type were distributed evenly on the surface of the chitosan sponges. Degradation test showed a significant effect of TiO2 nanoparticles addition in retaining its integrity. Biomineralization assay using simulated body fluid showed apatite formation in sponges surface as denoted by PO4− band observed in FTIR results. qPCR analysis supported chitosan - TiO2 sponges in bone regeneration capability as indicated by DMP1 and OCN gene upregulation in TiO2 treated group. Finally, cytotoxicity analysis supported the fact that TiO2 nanoparticles added sponges were proved to be biocompatible. Results suggest that chitosan-50% TiO2 nanoparticles sponges could be a potential novel scaffold for bone tissue engineering.

AB - Chitosan has been a popular option for tissue engineering, however exhibits limited function for bone regeneration due to its low mechanical robustness and non-osteogenic inductivity. Here we hybridized chitosan with TiO2 nanoparticles to improve its bone regeneration capability. Morphology and crystallographic analysis showed that TiO2 nanoparticles in anatase-type were distributed evenly on the surface of the chitosan sponges. Degradation test showed a significant effect of TiO2 nanoparticles addition in retaining its integrity. Biomineralization assay using simulated body fluid showed apatite formation in sponges surface as denoted by PO4− band observed in FTIR results. qPCR analysis supported chitosan - TiO2 sponges in bone regeneration capability as indicated by DMP1 and OCN gene upregulation in TiO2 treated group. Finally, cytotoxicity analysis supported the fact that TiO2 nanoparticles added sponges were proved to be biocompatible. Results suggest that chitosan-50% TiO2 nanoparticles sponges could be a potential novel scaffold for bone tissue engineering.

KW - Bone regeneration

KW - Chitosan

KW - Sponges

KW - TiO nanoparticles

KW - Tissue engineering

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DO - 10.1016/j.btre.2019.e00350

M3 - Article

AN - SCOPUS:85067517638

SN - 2215-017X

VL - 24

JO - Biotechnology Reports

JF - Biotechnology Reports

M1 - e00350

ER -

Enhanced bone regeneration capability of chitosan sponge coated with TiO₂ nanoparticles

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Keywords

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