Endothelial cell culture from human umbilical cord vein

Sri Bekti Subakir; Dewi Irawati Soeria Santoso; Minarma Siagian; I. G.A.F. Maging

doi:10.13181/mji.v3i2.944

Endothelial cell culture from human umbilical cord vein

Sri Bekti Subakir, Dewi Irawati Soeria Santoso, Minarma Siagian, I. G.A.F. Maging

Department of Physiology Pre Clinic

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Endothelial cells play an important role in various physiologic and pathologic processes. Their function in biologic events can be better understood if these endothelial cells can be isolated and cultured in vitro. The success of endothelial cell culture in the tropics depends upon controlling bacterial or fungal contamination. An attempt was made to isolate and culture endothelial cells from human umbilical cord vein using an adaptation of the method employed by Jaffe.⁴ Out of 50 umbilical cord vein samples cultured, 35 produced confluent cultures. It is hoped that this present trial will be the bases of further studies on the functions of endothelial cells in physiologic and pathologic processes.

Original language	English
Pages (from-to)	65-68
Number of pages	4
Journal	Medical Journal of Indonesia
Volume	3
Issue number	2
DOIs	https://doi.org/10.13181/mji.v3i2.944
Publication status	Published - 1 Apr 1994

Keywords

Bacterial and fungal contamination
Confluent growth
Endothelial cell isolation
Human umbilical vein endothelial cell culture

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title = "Endothelial cell culture from human umbilical cord vein",

abstract = "Endothelial cells play an important role in various physiologic and pathologic processes. Their function in biologic events can be better understood if these endothelial cells can be isolated and cultured in vitro. The success of endothelial cell culture in the tropics depends upon controlling bacterial or fungal contamination. An attempt was made to isolate and culture endothelial cells from human umbilical cord vein using an adaptation of the method employed by Jaffe.4 Out of 50 umbilical cord vein samples cultured, 35 produced confluent cultures. It is hoped that this present trial will be the bases of further studies on the functions of endothelial cells in physiologic and pathologic processes.",

keywords = "Bacterial and fungal contamination, Confluent growth, Endothelial cell isolation, Human umbilical vein endothelial cell culture",

author = "Subakir, {Sri Bekti} and Santoso, {Dewi Irawati Soeria} and Minarma Siagian and Maging, {I. G.A.F.}",

note = "Funding Information: This research is supported by the Special Programme of Research, Development and Research Training in Human Reproduction, World Health Organization. The authors wishes to express their gratitude to the Department of Pathology, Faculty of Medicine, University of Indonesia for the use of the tissue culture laboratory. Publisher Copyright: {\textcopyright} 1994, Faculty of Medicine, Universitas Indonesia. All rights reserved.",

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AU - Santoso, Dewi Irawati Soeria

AU - Siagian, Minarma

AU - Maging, I. G.A.F.

N1 - Funding Information: This research is supported by the Special Programme of Research, Development and Research Training in Human Reproduction, World Health Organization. The authors wishes to express their gratitude to the Department of Pathology, Faculty of Medicine, University of Indonesia for the use of the tissue culture laboratory. Publisher Copyright: © 1994, Faculty of Medicine, Universitas Indonesia. All rights reserved.

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Y1 - 1994/4/1

N2 - Endothelial cells play an important role in various physiologic and pathologic processes. Their function in biologic events can be better understood if these endothelial cells can be isolated and cultured in vitro. The success of endothelial cell culture in the tropics depends upon controlling bacterial or fungal contamination. An attempt was made to isolate and culture endothelial cells from human umbilical cord vein using an adaptation of the method employed by Jaffe.4 Out of 50 umbilical cord vein samples cultured, 35 produced confluent cultures. It is hoped that this present trial will be the bases of further studies on the functions of endothelial cells in physiologic and pathologic processes.

AB - Endothelial cells play an important role in various physiologic and pathologic processes. Their function in biologic events can be better understood if these endothelial cells can be isolated and cultured in vitro. The success of endothelial cell culture in the tropics depends upon controlling bacterial or fungal contamination. An attempt was made to isolate and culture endothelial cells from human umbilical cord vein using an adaptation of the method employed by Jaffe.4 Out of 50 umbilical cord vein samples cultured, 35 produced confluent cultures. It is hoped that this present trial will be the bases of further studies on the functions of endothelial cells in physiologic and pathologic processes.

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JO - Medical Journal of Indonesia

JF - Medical Journal of Indonesia

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Endothelial cell culture from human umbilical cord vein

Abstract

Keywords

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