Endothelial cells play an important role in various physiologic and pathologic processes. Their function in biologic events can be better understood if these endothelial cells can be isolated and cultured in vitro. The success of endothelial cell culture in the tropics depends upon controlling bacterial or fungal contamination. An attempt was made to isolate and culture endothelial cells from human umbilical cord vein using an adaptation of the method employed by Jaffe.4 Out of 50 umbilical cord vein samples cultured, 35 produced confluent cultures. It is hoped that this present trial will be the bases of further studies on the functions of endothelial cells in physiologic and pathologic processes.
- Bacterial and fungal contamination
- Confluent growth
- Endothelial cell isolation
- Human umbilical vein endothelial cell culture