Endometrial αvβ3 integrin expression in Macaca nemestrina endometrium after gonadotrophin administration for controlled ovarian hyperstimulation

Controlled ovarian hyperstimulation (COH) has a detrimental effect on endometrial reception during the implantation period. Here, this effect is estimated based on changes in αvβ3 integrin expression as an indicator of endometrial reception. Four groups of four Macaca nemestrina were randomly assigned to control and three treatment groups. The treatment group received gonadotrophin recombinant follicle stimulating hormone (rFSH) at 30, 50 and 70IU through a long protocol method. Blood collected before ovulation, on the day of hCG administration. Endometrial extraction is carried out in the midluteal phase, at 8-10 days after the highest peak of estradiol. Integrin expression of αvβ3 was assessed by immunihistochemistry and steroid hormone by the enzyme linked immunosorbent assay (Elisa) test. The result found that there was no significant difference between the control group αvβ3 integrin expression and the treatment group (P = 0.107). No significant relationship also found between the expression of αvβ3 integrins and estradiol and progesterone levels on the day of hCG administration. Compared with progesterone levels on the day of hCG secretion, the expression of αvβ3 integrin decreased with progesterone concentration > 6 ng / mL.