Embryo quality after intra-cytoplasmic sperm injection involving sperm associated with azoospermia and aged oocytes: A case report

Embryo quality determines the pregnancy rate and development of healthy offspring. Embryo profiling can help determine embryo quality through qualification and quantification of various parameters. Here we report a case of successful fertilization with intra-cytoplasmic sperm injection (ICSI) involving sperm associated with azoospermia and aged oocytes. Studies have reported success of ICSI involving sperm associated with azoospermia and aged oocytes, and the findings of the present report will fill the literature gap by providing evidence on the fertilization success rate with ICSI involving sperm associated with azoospermia. A 39-year-old man and 35-year-old woman visited our infertility center. ICSI was performed, and embryos were obtained. Two embryos were freshly transferred on day 3, and two embryos were frozen. As the fresh embryo transfer failed, the frozen embryos were used for frozen embryo transfer, and pregnancy was successfully achieved. We believe that the DNA quality of the testicular sperm determines the pregnancy success rate. Although moderate-quality embryos in frozen embryo transfer were exposed to freezing effects, pregnancy was successfully achieved, possibly because of better receptivity of the endometrium.