Electrogenerated Chemiluminescence for Immunoassay Applications

Isnaini Rahmawati; Irkham Irkham; Rahmat Wibowo; Jarnuzi Gunlazuardi; Yasuaki Einaga; Tribidasari Anggraningrum Ivandini

doi:10.22146/IJC.64596

Electrogenerated Chemiluminescence for Immunoassay Applications

Isnaini Rahmawati, Irkham Irkham, Rahmat Wibowo, Jarnuzi Gunlazuardi, Yasuaki Einaga, Tribidasari Anggraningrum Ivandini

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Electrogenerated chemiluminescence (ECL) has recently become one of the most prominent and well-established transducers for immunoassay techniques. ECL relates a luminophore concentration in solution with the emission of light triggered by an electrochemical stimulus. ECL immunoassay (ECLIA) performance depends on the parameters of its light generation, including the luminophore, the species that emit light called labels in ECLIA; co-reactants, which are added reagents that support the luminophore to undergo the excited state; electrodes, which are the place for the ECL reactions to take place; and the format of the immunoassay. This review discusses the behaviour of ECLIA parameters, the required instrumentations, and some important examples of detections based on ECLIA.

Original language	English
Pages (from-to)	1599-1618
Number of pages	20
Journal	Indonesian Journal of Chemistry
Volume	21
Issue number	6
DOIs	https://doi.org/10.22146/IJC.64596
Publication status	Published - 2021

Keywords

Antibody
Antigen
ECL
ECLIA
Electrochemiluminescence
Immunoassay

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10.22146/IJC.64596

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title = "Electrogenerated Chemiluminescence for Immunoassay Applications",

abstract = "Electrogenerated chemiluminescence (ECL) has recently become one of the most prominent and well-established transducers for immunoassay techniques. ECL relates a luminophore concentration in solution with the emission of light triggered by an electrochemical stimulus. ECL immunoassay (ECLIA) performance depends on the parameters of its light generation, including the luminophore, the species that emit light called labels in ECLIA; co-reactants, which are added reagents that support the luminophore to undergo the excited state; electrodes, which are the place for the ECL reactions to take place; and the format of the immunoassay. This review discusses the behaviour of ECLIA parameters, the required instrumentations, and some important examples of detections based on ECLIA.",

keywords = "Antibody, Antigen, ECL, ECLIA, Electrochemiluminescence, Immunoassay",

author = "Isnaini Rahmawati and Irkham Irkham and Rahmat Wibowo and Jarnuzi Gunlazuardi and Yasuaki Einaga and Ivandini, {Tribidasari Anggraningrum}",

note = "Funding Information: The authors would like to acknowledge the financial support provided by Directorate General of Higher Education, Ministry of Education and Culture, Republic of Indonesia via PMDSU scholarship, Grant No. NKB-432/UN2.RST/HKP.05.00/2020. Publisher Copyright: {\textcopyright} 2021, Gadjah Mada University. All rights reserved.",

year = "2021",

doi = "10.22146/IJC.64596",

language = "English",

volume = "21",

pages = "1599--1618",

journal = "Indonesian Journal of Chemistry",

issn = "1411-9420",

publisher = "Gadjah Mada University",

number = "6",

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TY - JOUR

T1 - Electrogenerated Chemiluminescence for Immunoassay Applications

AU - Rahmawati, Isnaini

AU - Irkham, Irkham

AU - Wibowo, Rahmat

AU - Gunlazuardi, Jarnuzi

AU - Einaga, Yasuaki

AU - Ivandini, Tribidasari Anggraningrum

N1 - Funding Information: The authors would like to acknowledge the financial support provided by Directorate General of Higher Education, Ministry of Education and Culture, Republic of Indonesia via PMDSU scholarship, Grant No. NKB-432/UN2.RST/HKP.05.00/2020. Publisher Copyright: © 2021, Gadjah Mada University. All rights reserved.

PY - 2021

Y1 - 2021

N2 - Electrogenerated chemiluminescence (ECL) has recently become one of the most prominent and well-established transducers for immunoassay techniques. ECL relates a luminophore concentration in solution with the emission of light triggered by an electrochemical stimulus. ECL immunoassay (ECLIA) performance depends on the parameters of its light generation, including the luminophore, the species that emit light called labels in ECLIA; co-reactants, which are added reagents that support the luminophore to undergo the excited state; electrodes, which are the place for the ECL reactions to take place; and the format of the immunoassay. This review discusses the behaviour of ECLIA parameters, the required instrumentations, and some important examples of detections based on ECLIA.

AB - Electrogenerated chemiluminescence (ECL) has recently become one of the most prominent and well-established transducers for immunoassay techniques. ECL relates a luminophore concentration in solution with the emission of light triggered by an electrochemical stimulus. ECL immunoassay (ECLIA) performance depends on the parameters of its light generation, including the luminophore, the species that emit light called labels in ECLIA; co-reactants, which are added reagents that support the luminophore to undergo the excited state; electrodes, which are the place for the ECL reactions to take place; and the format of the immunoassay. This review discusses the behaviour of ECLIA parameters, the required instrumentations, and some important examples of detections based on ECLIA.

KW - Antibody

KW - Antigen

KW - ECL

KW - ECLIA

KW - Electrochemiluminescence

KW - Immunoassay

UR - http://www.scopus.com/inward/record.url?scp=85123763638&partnerID=8YFLogxK

U2 - 10.22146/IJC.64596

DO - 10.22146/IJC.64596

M3 - Article

AN - SCOPUS:85123763638

SN - 1411-9420

VL - 21

SP - 1599

EP - 1618

JO - Indonesian Journal of Chemistry

JF - Indonesian Journal of Chemistry

IS - 6

ER -

Electrogenerated Chemiluminescence for Immunoassay Applications

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Keywords

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