Electrochemical detection of tricyclic antidepressant drugs by HPLC using highly boron-doped diamond electrodes

Ivandini Tribidasari Anggraningrum, B. V. Sarada, C. Terashima, T. N. Rao, D. A. Tryk, H. Ishiguro, Y. Kubota, A. Fujishima

Research output: Contribution to journalArticlepeer-review

124 Citations (Scopus)

Abstract

Boron-doped diamond (BDD) electrodes have been examined for the electrochemical detection of six tricyclic antidepressant drugs (TCAs): Imipramine, desipramine, clomipramine, amitriptyline, nortriptyline, and doxepin. Cyclic voltammetry, flow injection analysis (FIA) and HPLC with electrochemical detection have been used to study the oxidation reactions and to detect the TCAs. At diamond electrodes, well-defined and highly reproducible voltammograms were obtained for all six drugs with a signal to background (S/B) ratio about 2-4 times greater than those at glassy carbon electrodes. Diamond is the first electrode material to show well-defined voltammograms for nortriptyline due to its wide potential window. In the FIA-mode, at an operation potential of 0.85 V versus Ag AgCl, diamond exhibited a background current of 7 nA with rapid stabilization (15 min) conversely to the case of GC, which appeared to stabilize after 1 h, but again increased thereafter. The analytical peaks of HPLC for the TCAs were well resolved. Linear calibration curves were linear over the ranges from 0.05 to 100 μM. The limits of detection (S/N=3) were 3 nM for imipramine and desipramine, 0.5 nM for clomipramine, 163 nM amitriptyline, 1080 nM for nortriptyline and 92 nM for doxepin. The electrodes have shown reproducible results over several days of analysis. This method has been applied for the determination of imipramine and desipramine in plasma samples. The BDD surface was reproducible with no adsorption of blood components during plasma analysis. This work shows the promising use of diamond as an amperometric detector in HPLC, especially for TCA analysis.

Original languageEnglish
Pages (from-to)117-126
Number of pages10
JournalJournal of Electroanalytical Chemistry
Volume521
Issue number1-2
DOIs
Publication statusPublished - 8 Mar 2002

Keywords

  • Boron-doped diamond
  • HPLC
  • Plasma sample
  • Reproducibility
  • Tricyclic antidepressant drugs

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