Ekspresi tissue inhibitor metaloproteinase-2 pada metastasis kelenjar limfe leher pada karsinoma sel skuamosa laring

Background: Survival of laryngeal cancer cases depend on lymph nodes involvement. Tissue inhibitormetaloproteinase-2 (TIMP-2) has double effects on matrix metalloproteinase. TIMP-2 could inhibit orinduce metastasis process. Its expression depends on tumor location and histopathology. Purpose: Tofind out the presence of TIMP-2 on biopsy specimens of laryngeal squamous cell carcinoma and itsrelation to lymph node involvement. Methods: The immunoexpression of TIMP-2 was studied on biopsy specimens of 15 laryngeal carcinomas with lymph node metastasis and 18 patients without, collected between 2005-2009. Immunohistochemistry test were carried out in Pathology Department Universityof Indonesia using TIMP-2 (3A4) sc 21735 antibody from Santa Cruz. We used analytic-descriptivestudy to compare TIMP-2 expression between laryngeal squamous cell carcinoma with lymph node metastasis and those without. Results: The median of TIMP-2 immunoexpression in laryngeal squamouscell carcinoma in patients with lymph node involvement was 99 and the median in patients withoutlymph node metastases was 97, with range between 0-100. Statistical analysis revealed no significantdifference in TIMP-2 expression between groups with metastasis and without (p=0.403). Conclusions:Our results could not differentiate the TIMP-2 expression from patients with laryngeal squamous cellcarcinoma with lymph node metastasis and without. However, imbalances in the extracellular activitiesof matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases, linked to pathologicaltissue destruction, are more complex and need further investigation. Further study on correlationbetween TIMP-2 and MMP2-MT-1MMP immunoexpression is advised to determine its relationship tolymph node metastasis and to plan more aggressive treatment.