Efficacy of propolis fluoride in inhibiting the formation of Streptoccocus mutans, Streptococcus gordonii, and Streptococcus sanguinis biofilm

Sri Angky Soekanto, Saly Salim Alatas, Rima Ristanti, Ferry P. Gultom, Muhamad Sahlan

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The objective of this study was to analyze the effectiveness of Propolis Fluoride in inhibiting the biofilm formation of S. mutans, S. gordonii and S. sanguinis in comparison to Silver Diamine Fluoride. Bacteria suspensions were cultured in Brain Heart Infusion Broth, then dripped onto a 96-well plate. Propolis fluoride was added to the well plates, which were then incubated for 4 hours, 12 hours, 24 hours, and 48 hours. Afterwards, Crystal Violet 0.5% was added to each well plate, and a micro plate reader was used to evaluate the Optical Density value at 570nm wavelength. S. gordonii and S. mutans were analyzed using MTT assay by inserting the bacterial suspensions into a BHI medium enriched with 0.2% sucrose and exposed to PPF, then incubated at 4 different times. The ANOVA test result on the potential of Propolis Fluoride to inhibit biofilm formation of S. mutans, S. gordonii and S. sanguinis was equal to 38%.(b/b) Silver Diamine Fluoride, Propolis Fluoride has the same inhibition potential and effectiveness against biofilm formation as Silver Diamine Fluoride.

Original languageEnglish
Title of host publication3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2018
EditorsPraswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418226
DOIs
Publication statusPublished - 9 Apr 2019
Event3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia
Duration: 6 Aug 20188 Aug 2018

Publication series

NameAIP Conference Proceedings
Volume2092
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
CountryIndonesia
CityJakarta
Period6/08/188/08/18

Keywords

  • Propolis fluoride
  • S. mutans
  • S. sanguinis, S. gordonii
  • silver diamine fluoride

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