Efficacy of propolis fluoride in inhibiting the formation of Streptoccocus mutans, Streptococcus gordonii, and Streptococcus sanguinis biofilm

Sri Angky Soekanto; Saly Salim Alatas; Rima Ristanti; Ferry P. Gultom; Muhamad Sahlan

doi:10.1063/1.5096712

Efficacy of propolis fluoride in inhibiting the formation of Streptoccocus mutans, Streptococcus gordonii, and Streptococcus sanguinis biofilm

Sri Angky Soekanto, Saly Salim Alatas, Rima Ristanti, Ferry P. Gultom, Muhamad Sahlan

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

The objective of this study was to analyze the effectiveness of Propolis Fluoride in inhibiting the biofilm formation of S. mutans, S. gordonii and S. sanguinis in comparison to Silver Diamine Fluoride. Bacteria suspensions were cultured in Brain Heart Infusion Broth, then dripped onto a 96-well plate. Propolis fluoride was added to the well plates, which were then incubated for 4 hours, 12 hours, 24 hours, and 48 hours. Afterwards, Crystal Violet 0.5% was added to each well plate, and a micro plate reader was used to evaluate the Optical Density value at 570nm wavelength. S. gordonii and S. mutans were analyzed using MTT assay by inserting the bacterial suspensions into a BHI medium enriched with 0.2% sucrose and exposed to PPF, then incubated at 4 different times. The ANOVA test result on the potential of Propolis Fluoride to inhibit biofilm formation of S. mutans, S. gordonii and S. sanguinis was equal to 38%.(b/b) Silver Diamine Fluoride, Propolis Fluoride has the same inhibition potential and effectiveness against biofilm formation as Silver Diamine Fluoride.

Original language	English
Title of host publication	3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publication	Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018
Editors	Praswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735418226
DOIs	https://doi.org/10.1063/1.5096712
Publication status	Published - 9 Apr 2019
Event	3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia Duration: 6 Aug 2018 → 8 Aug 2018

Publication series

Name	AIP Conference Proceedings
Volume	2092
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
Country/Territory	Indonesia
City	Jakarta
Period	6/08/18 → 8/08/18

Keywords

Propolis fluoride
S. mutans
S. sanguinis, S. gordonii
silver diamine fluoride

Access to Document

10.1063/1.5096712

Cite this

Soekanto, S. A., Alatas, S. S., Ristanti, R., Gultom, F. P., & Sahlan, M. (2019). Efficacy of propolis fluoride in inhibiting the formation of Streptoccocus mutans, Streptococcus gordonii, and Streptococcus sanguinis biofilm. In P. P. D. K. Wulan, M. Gozan, S. Astutiningsih, G. Ramahdita, R. Dhelika, & P. Kreshanti (Eds.), 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018 Article 030008 (AIP Conference Proceedings; Vol. 2092). American Institute of Physics Inc.. https://doi.org/10.1063/1.5096712

Soekanto, Sri Angky ; Alatas, Saly Salim ; Ristanti, Rima et al. / Efficacy of propolis fluoride in inhibiting the formation of Streptoccocus mutans, Streptococcus gordonii, and Streptococcus sanguinis biofilm. 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. editor / Praswasti P.D.K. Wulan ; Misri Gozan ; Sotya Astutiningsih ; Ghiska Ramahdita ; Radon Dhelika ; Prasetyanugraheni Kreshanti. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).

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title = "Efficacy of propolis fluoride in inhibiting the formation of Streptoccocus mutans, Streptococcus gordonii, and Streptococcus sanguinis biofilm",

abstract = "The objective of this study was to analyze the effectiveness of Propolis Fluoride in inhibiting the biofilm formation of S. mutans, S. gordonii and S. sanguinis in comparison to Silver Diamine Fluoride. Bacteria suspensions were cultured in Brain Heart Infusion Broth, then dripped onto a 96-well plate. Propolis fluoride was added to the well plates, which were then incubated for 4 hours, 12 hours, 24 hours, and 48 hours. Afterwards, Crystal Violet 0.5% was added to each well plate, and a micro plate reader was used to evaluate the Optical Density value at 570nm wavelength. S. gordonii and S. mutans were analyzed using MTT assay by inserting the bacterial suspensions into a BHI medium enriched with 0.2% sucrose and exposed to PPF, then incubated at 4 different times. The ANOVA test result on the potential of Propolis Fluoride to inhibit biofilm formation of S. mutans, S. gordonii and S. sanguinis was equal to 38%.(b/b) Silver Diamine Fluoride, Propolis Fluoride has the same inhibition potential and effectiveness against biofilm formation as Silver Diamine Fluoride.",

keywords = "Propolis fluoride, S. mutans, S. sanguinis, S. gordonii, silver diamine fluoride",

author = "Soekanto, {Sri Angky} and Alatas, {Saly Salim} and Rima Ristanti and Gultom, {Ferry P.} and Muhamad Sahlan",

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doi = "10.1063/1.5096712",

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Soekanto, SA, Alatas, SS, Ristanti, R, Gultom, FP & Sahlan, M 2019, Efficacy of propolis fluoride in inhibiting the formation of Streptoccocus mutans, Streptococcus gordonii, and Streptococcus sanguinis biofilm. in PPDK Wulan, M Gozan, S Astutiningsih, G Ramahdita, R Dhelika & P Kreshanti (eds), 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018., 030008, AIP Conference Proceedings, vol. 2092, American Institute of Physics Inc., 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018, Jakarta, Indonesia, 6/08/18. https://doi.org/10.1063/1.5096712

Efficacy of propolis fluoride in inhibiting the formation of Streptoccocus mutans, Streptococcus gordonii, and Streptococcus sanguinis biofilm. / Soekanto, Sri Angky; Alatas, Saly Salim; Ristanti, Rima et al.
3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. ed. / Praswasti P.D.K. Wulan; Misri Gozan; Sotya Astutiningsih; Ghiska Ramahdita; Radon Dhelika; Prasetyanugraheni Kreshanti. American Institute of Physics Inc., 2019. 030008 (AIP Conference Proceedings; Vol. 2092).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Soekanto, Sri Angky

AU - Alatas, Saly Salim

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AU - Gultom, Ferry P.

AU - Sahlan, Muhamad

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N2 - The objective of this study was to analyze the effectiveness of Propolis Fluoride in inhibiting the biofilm formation of S. mutans, S. gordonii and S. sanguinis in comparison to Silver Diamine Fluoride. Bacteria suspensions were cultured in Brain Heart Infusion Broth, then dripped onto a 96-well plate. Propolis fluoride was added to the well plates, which were then incubated for 4 hours, 12 hours, 24 hours, and 48 hours. Afterwards, Crystal Violet 0.5% was added to each well plate, and a micro plate reader was used to evaluate the Optical Density value at 570nm wavelength. S. gordonii and S. mutans were analyzed using MTT assay by inserting the bacterial suspensions into a BHI medium enriched with 0.2% sucrose and exposed to PPF, then incubated at 4 different times. The ANOVA test result on the potential of Propolis Fluoride to inhibit biofilm formation of S. mutans, S. gordonii and S. sanguinis was equal to 38%.(b/b) Silver Diamine Fluoride, Propolis Fluoride has the same inhibition potential and effectiveness against biofilm formation as Silver Diamine Fluoride.

AB - The objective of this study was to analyze the effectiveness of Propolis Fluoride in inhibiting the biofilm formation of S. mutans, S. gordonii and S. sanguinis in comparison to Silver Diamine Fluoride. Bacteria suspensions were cultured in Brain Heart Infusion Broth, then dripped onto a 96-well plate. Propolis fluoride was added to the well plates, which were then incubated for 4 hours, 12 hours, 24 hours, and 48 hours. Afterwards, Crystal Violet 0.5% was added to each well plate, and a micro plate reader was used to evaluate the Optical Density value at 570nm wavelength. S. gordonii and S. mutans were analyzed using MTT assay by inserting the bacterial suspensions into a BHI medium enriched with 0.2% sucrose and exposed to PPF, then incubated at 4 different times. The ANOVA test result on the potential of Propolis Fluoride to inhibit biofilm formation of S. mutans, S. gordonii and S. sanguinis was equal to 38%.(b/b) Silver Diamine Fluoride, Propolis Fluoride has the same inhibition potential and effectiveness against biofilm formation as Silver Diamine Fluoride.

KW - Propolis fluoride

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Soekanto SA, Alatas SS, Ristanti R, Gultom FP, Sahlan M. Efficacy of propolis fluoride in inhibiting the formation of Streptoccocus mutans, Streptococcus gordonii, and Streptococcus sanguinis biofilm. In Wulan PPDK, Gozan M, Astutiningsih S, Ramahdita G, Dhelika R, Kreshanti P, editors, 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. American Institute of Physics Inc. 2019. 030008. (AIP Conference Proceedings). doi: 10.1063/1.5096712

Efficacy of propolis fluoride in inhibiting the formation of Streptoccocus mutans, Streptococcus gordonii, and Streptococcus sanguinis biofilm

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