TY - JOUR
T1 - Effects of intravenous palm oil-based lipid nanoemulsion on fat metabolism in rabbits
AU - Jufri, Mahdi
AU - Azmi, Norazrina
AU - Shamsuddin, Ahmad Fuad
N1 - Funding Information:
The authors would like to thank the Malaysian Technology Development Corporation for the research grant (MTDC-BF0003-2008) and the Faculty of Pharmacy, UKM where the research was conducted. Also to Prof. Baharudin Hj. Omar, from Dept. of Epidemiology, Faculty of Health Science, UKM who helped in the statistical analysis and Prof. Dr. Srijit Das (Dept. of Anatomy, Faculty of Medicine, UKM) who has helped in editing the manuscript.
Funding Information:
*Corresponding author: Ahmad Fuad Shamsuddin, Faculty of Pharmacy, Universiti Kebangsaan Malaysia , Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. Tel : +6 03 92897315 Fax : + 6 03 26983271 E-mail: [email protected] Fundation Project: Supported by The Malaysian Technology Development Corporation (Grant No: UKM-MTDC-BF0003-2008)
PY - 2012
Y1 - 2012
N2 - Objective: To investigate the effects of a newly-developed nanoemulsion of palm oil in combination with medium chain triglyceride (MCT) oil (NEMS®MCT/LCT20%) on fat metabolism in male New Zealand white rabbits. Methods: Six rabbits were divided into two groups of three rabbits in each group. NEMS®MCT/LCT20%, Lipofundin®MCT/LCT 20% (a commercially-available lipid emulsion) and normal saline (control) were administered intravenously in these rabbits via the marginal ear vein for 6 h. Cross-over method was used in these rabbits with a wash-out period of two weeks in-between infusions. Triglyceride (TG), total cholesterol, low density lipoprotein and high density lipoprotein cholesterols were determined using enzymatic strip test at 0, 2, 4 and 6 h of infusion. Blood levels of free fatty acid (FFA) were measured at 0 and 6 h. Results: Serum TG levels increased in these rabbits after 2 and 4 h infusion of NEMS®MCT/LCT20% but returned to normal after 6 h. Concentration of FFA increased in a dose-related manner but remained within the normal range (315-535 μmol/L). Similar results were obtained with Lipofundin®MCT/LCT20%. All parameters of fat metabolism in these rabbits remained unchanged from baseline when normal saline was administered. Changes in the parameters of fat metabolism measured between NEMS®MCT/LCT20% and Lipofundin®MCT/LCT20% did not show any significant difference. Significant difference (P<0.05) was observed in these parameters when NEMS®MCT/LCT20% was compared to normal saline. Conclusions: Intravenous administration of NEMS®MCT/LCT20% did not cause a permanent increase in the TG level of rabbits while FFA remained within the normal range.
AB - Objective: To investigate the effects of a newly-developed nanoemulsion of palm oil in combination with medium chain triglyceride (MCT) oil (NEMS®MCT/LCT20%) on fat metabolism in male New Zealand white rabbits. Methods: Six rabbits were divided into two groups of three rabbits in each group. NEMS®MCT/LCT20%, Lipofundin®MCT/LCT 20% (a commercially-available lipid emulsion) and normal saline (control) were administered intravenously in these rabbits via the marginal ear vein for 6 h. Cross-over method was used in these rabbits with a wash-out period of two weeks in-between infusions. Triglyceride (TG), total cholesterol, low density lipoprotein and high density lipoprotein cholesterols were determined using enzymatic strip test at 0, 2, 4 and 6 h of infusion. Blood levels of free fatty acid (FFA) were measured at 0 and 6 h. Results: Serum TG levels increased in these rabbits after 2 and 4 h infusion of NEMS®MCT/LCT20% but returned to normal after 6 h. Concentration of FFA increased in a dose-related manner but remained within the normal range (315-535 μmol/L). Similar results were obtained with Lipofundin®MCT/LCT20%. All parameters of fat metabolism in these rabbits remained unchanged from baseline when normal saline was administered. Changes in the parameters of fat metabolism measured between NEMS®MCT/LCT20% and Lipofundin®MCT/LCT20% did not show any significant difference. Significant difference (P<0.05) was observed in these parameters when NEMS®MCT/LCT20% was compared to normal saline. Conclusions: Intravenous administration of NEMS®MCT/LCT20% did not cause a permanent increase in the TG level of rabbits while FFA remained within the normal range.
KW - Fat metabolism
KW - Free fatty acid
KW - Intravenous
KW - Lipofundin®MCT/LCT20%
KW - Medium chain triglyceride
KW - NEMS®MCT/LCT20%
KW - Nanoemulsion
KW - Palm oil
KW - Serum triglyceride
UR - http://www.scopus.com/inward/record.url?scp=84871792940&partnerID=8YFLogxK
U2 - 10.1016/S2222-1808(12)60297-7
DO - 10.1016/S2222-1808(12)60297-7
M3 - Article
AN - SCOPUS:84871792940
SN - 2222-1808
VL - 2
SP - S944-S948
JO - Asian Pacific Journal of Tropical Disease
JF - Asian Pacific Journal of Tropical Disease
IS - SUPPL2
ER -
