Effects of intestinal strangulation release on liver following intestinal ischemia-reperfusion injury on Sprague Dawley rat

BACKGROUND: Intestinal strangulation often causes ischemia-reperfusion injury (IRI) on the liver as a remote organ that directly gets blood flow from the intestine through the portal vein. Intestinal strangulation release to restore oxygen and evaluate the viability of intestine tissue can cause IRIthat aggravates the cellular injury. This research aimed to investigate the effects of intestinal strangulation release on the liver following intestinal ischemia-reperfusion injury. METHODS: Experimental research was done using fourteen Sprague-Dawley rats to compare aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum malondialdehyde (MDA), liver MDAlevels, and histopathological liver injury degree between the control group, strangulation release group, and without strangulation release group after 4 hours of one loop ileum strangulation. The strangulation was a 10 cm length loop of the ileum. Moreover, the strangulation release was conducted 4 hours after each strangulation. Blood samples were for AST, ALT, and MDA levels examinations were taken 4 hours after strangulation releases. RESULTS: There was an insignificant difference in AST (P=0.234), ALT (P=0.458), serum MDA (P=0.646), and liver MDA (P=0.237) levels between the three groups. Liver histopathology examination showed a significant difference between control and both treatment groups (P=0.021), but an insignificant difference between the release group and without release group (P=0.902). RESULTS: Intestinal strangulation release right before resection to evaluate the viability and lessen the length of resected intestine on critical intestinal ischemia does not cause significant differences of effects on the liver.