TY - JOUR
T1 - Effects of C. ramiflora Linn. leaf extract against dengue virus replication in vitro on Huh7it-1 cell
AU - Meutia, Zatuilla Zahra
AU - Dewi, Beti Ernawati
N1 - Publisher Copyright:
© 2017 American Scientific Publishers All rights reserved.
PY - 2017/7
Y1 - 2017/7
N2 - Introduction: Dengue virus (DENV) causes an acute infection that may lead to death. Due to the morbidity and mortality produced, dengue infection is still a serious health problem especially in tropical countries. To this time, there is still no specific antiviral to overcome DENV infection. Therefore, a research to find a specific antiviral for DENV is necessary. This research is aimed to evaluate the effect of Cynometra ramiflora Linn. leaf extract in vitro on Huh7it-1 cell as a specific antiviral for DENV infection. Methods: An experimental research was conducted in August 2014–June 2015. The leaf extract of C. ramiflora Linn. was diluted to concentration 40 μg/ml, 20 μg/ml, 10 μg/ml, 5 μg/ml, 2,5 μg/ml, and 1,25 μg/ml. Next, DENV was exposed to each concentration. The inhibition of DENV replication (IC50) was observed using Focus Assay, while the toxicity of the extract to Huh7it-1 (CC50) was evaluated using MTT Assay. Data were analyzed using T -test and One Way ANOVA. Results: From the experiment, the value of CC50 and IC50 are 125 μg/ml and 20,1 μg/ml, respectively. Conclusion: From the research, it is concluded that C. ramiflora Linn. extract is not toxic and has a potency as antiviral for DENV.
AB - Introduction: Dengue virus (DENV) causes an acute infection that may lead to death. Due to the morbidity and mortality produced, dengue infection is still a serious health problem especially in tropical countries. To this time, there is still no specific antiviral to overcome DENV infection. Therefore, a research to find a specific antiviral for DENV is necessary. This research is aimed to evaluate the effect of Cynometra ramiflora Linn. leaf extract in vitro on Huh7it-1 cell as a specific antiviral for DENV infection. Methods: An experimental research was conducted in August 2014–June 2015. The leaf extract of C. ramiflora Linn. was diluted to concentration 40 μg/ml, 20 μg/ml, 10 μg/ml, 5 μg/ml, 2,5 μg/ml, and 1,25 μg/ml. Next, DENV was exposed to each concentration. The inhibition of DENV replication (IC50) was observed using Focus Assay, while the toxicity of the extract to Huh7it-1 (CC50) was evaluated using MTT Assay. Data were analyzed using T -test and One Way ANOVA. Results: From the experiment, the value of CC50 and IC50 are 125 μg/ml and 20,1 μg/ml, respectively. Conclusion: From the research, it is concluded that C. ramiflora Linn. extract is not toxic and has a potency as antiviral for DENV.
KW - CC50
KW - Cynometra ramiflora linn
KW - DENV
KW - IC50
UR - http://www.scopus.com/inward/record.url?scp=85030249872&partnerID=8YFLogxK
U2 - 10.1166/asl.2017.9411
DO - 10.1166/asl.2017.9411
M3 - Article
AN - SCOPUS:85030249872
SN - 1936-6612
VL - 23
SP - 6834
EP - 6837
JO - Advanced Science Letters
JF - Advanced Science Letters
IS - 7
ER -