TY - JOUR
T1 - Effects of a probiotic milk drink on salivary tumor necrosis factor-alpha in children with acute lymphoblastic leukemia in the induction phase
AU - Pariaputra, Jennifer Surja
AU - Budiardjo, Sarworini B.
AU - Soetopo, Margaretha Suharsini
N1 - Funding Information:
The Publication of the manuscript is supported by Universitas Indonesia in 2017. This study was designed, conducted, and coordinated by Professor Sarworini B. Budiardjo and Professor Margaretha Suharsini as the principal investigator, provided conceptual and ethical guidance for all aspect of the project. Jennifer Surja Pariaputra performed, analyzed the data, and write the manuscript.
Publisher Copyright:
© 2018 The Authors.
PY - 2018/2
Y1 - 2018/2
N2 - Objective: The aim of this study was to investigate the effects of the short-term use of a probiotic milk drink on salivary tumor necrosis factor-alpha (TNF-α) in children with acute lymphoblastic leukemia (ALL) during the induction phase of chemotherapy. Methods: A randomized, case-controlled clinical trial was carried out. The samples of the study were collected from 11 children with ALL and 11 healthy children as the control. Both groups received a probiotic milk drink. The probiotic milk drink contained Lactobacillus casei (6.5×109 colony forming unit/mL). The subjects were instructed to rinse 5 mL of probiotic milk drink for 30 s over the course of 2 weeks. Unstimulated saliva samples were collected from patients at baseline and 1 and 2 weeks after rinsing with the probiotic milk drink. The levels of TNF-α were determined using a human TNF-α enzyme-linked immunosorbent assay kit. Results: The salivary TNF-α levels in the experimental group decreased but not to a statistically significant degree (p>0.05) when comparing the baseline with weeks 1 and 2 after rinsing. There was a significant decrease of salivary TNF-α levels in the control group (p<0.05) when comparing the baseline with 1 and 2 weeks after rinsing. The concentration of salivary TNF-α in the experimental group was higher than in the control group (p<0.05). Conclusion: This study demonstrates the effect of a short-term intake of a probiotic milk drink on children with ALL during the induction phase of chemotherapy. The results show a reduction of salivary TNF-α after taking the probiotic milk drink after 1 week and 2 weeks, although the results were not statistically significant.
AB - Objective: The aim of this study was to investigate the effects of the short-term use of a probiotic milk drink on salivary tumor necrosis factor-alpha (TNF-α) in children with acute lymphoblastic leukemia (ALL) during the induction phase of chemotherapy. Methods: A randomized, case-controlled clinical trial was carried out. The samples of the study were collected from 11 children with ALL and 11 healthy children as the control. Both groups received a probiotic milk drink. The probiotic milk drink contained Lactobacillus casei (6.5×109 colony forming unit/mL). The subjects were instructed to rinse 5 mL of probiotic milk drink for 30 s over the course of 2 weeks. Unstimulated saliva samples were collected from patients at baseline and 1 and 2 weeks after rinsing with the probiotic milk drink. The levels of TNF-α were determined using a human TNF-α enzyme-linked immunosorbent assay kit. Results: The salivary TNF-α levels in the experimental group decreased but not to a statistically significant degree (p>0.05) when comparing the baseline with weeks 1 and 2 after rinsing. There was a significant decrease of salivary TNF-α levels in the control group (p<0.05) when comparing the baseline with 1 and 2 weeks after rinsing. The concentration of salivary TNF-α in the experimental group was higher than in the control group (p<0.05). Conclusion: This study demonstrates the effect of a short-term intake of a probiotic milk drink on children with ALL during the induction phase of chemotherapy. The results show a reduction of salivary TNF-α after taking the probiotic milk drink after 1 week and 2 weeks, although the results were not statistically significant.
KW - Acute lymphoblastic leukemia
KW - Gingivitis
KW - Probiotics
KW - Salivary tumor necrosis factor-alpha
UR - http://www.scopus.com/inward/record.url?scp=85041669308&partnerID=8YFLogxK
U2 - 10.22159/ajpcr.2018.v11i2.23242
DO - 10.22159/ajpcr.2018.v11i2.23242
M3 - Article
AN - SCOPUS:85041669308
SN - 0974-2441
VL - 11
SP - 340
EP - 343
JO - Asian Journal of Pharmaceutical and Clinical Research
JF - Asian Journal of Pharmaceutical and Clinical Research
IS - 2
ER -