The present research was conducted to investigate the effect of extender (Marine Fish Ringer, MFR) and a combination of MFR and palm date solution on spermatozoa quality of giant grouper (E. lanceolatus), two days after sub-zero freezing. The sperm was collected and diluted either with MFR or MFR + 6 % glycerol + palm date. Palm date concentration used in this study were: 5 %, 10 %, 15 %, 20 % and 25 %, respectively. Sperm was then conditioned at 4 °C for 10 min and was refrigerated at -20 °C for two days. Thawing was conducted at 45 °C for 30 second. Data was analyzed either by ANOVA test or Tukey test. According to the ANOVA test, there was a significant impact (P > 0.05) of various concentrations of palm date on post-thaw sperm motility, compared to control (0 % of palm date). In accordance with the Turkey test, the concentration of 10 % of palm date indicated the highest post-thaw spermatozoa motility (76.70 ± 1.54 %) compared with MFR (68.03 ± 2.76 %). Our finding showed that 10 % of palm date was the optimum concentration.