Objective: This research objective is to evaluate the different types of anticoagulant to ethinyl estradiol and levonorgestrel analysis in plasma using ultra performance liquid chromatography tandem mass spectrometry. Method: Chromatography condition was obtained with Acquity® UPLC BEH C18 column (1.7 μm; 50 x 2.1 mm); mobile phase consisting 0.1% formic acid in water-acetonitrile under gradient elution ; flow rate of 0.3 mL/minute; column temperature of 40°C; injection volume of 10.0 μL; 5-minute analysis time and prednisone as internal standard. Sample preparation used protein precipitation followed by liquid-liquid extraction. Results: There was a linear result ranging from 5-500 pg/mL concentration of ethinyl estradiol and 100-10, 000 pg/ml concentration of levonorgestrel. There was no significant difference for stability and recovery of ethinyl estradiol and levonorgestrel in citrate, heparin, and EDTA plasma (p > 0.05; ANOVA). However, significant difference for peak area ratio (p < 0.05; Kruskal Wallis), between citrate, EDTA, and heparin plasma was observed. Conclusion: Citrate and heparin plasma analysis had better result than EDTA plasma analysis.
|Number of pages||9|
|Journal||Journal of Global Pharma Technology|
|Publication status||Published - 1 Jan 2018|
- Ethinyl estradiol