Effect of triple antibiotic paste, calcium hydroxide, ledermix® on viability of pulp mesenchymal stem cells

Emiria Dita Prasanti, Anggraini Margono, Nilakesuma Djauharie

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Objective: The goal of regenerative endodontic therapy is biological healing of the pulp tissue. It involves the disinfection of the canals with irrigants and medicaments. The medicaments that are currently used for this purpose are a triple antibiotic paste (TAP), calcium hydroxide (Ca[OH]2), and Ledermix®, a paste containing demeclocycline and triamcinolone. Therefore, the purpose of this study was to evaluate the effects of TAP, Ca(OH)2, and Ledermix® on the viability of dental pulp stem cells (DPSC). Methods: Primary cultures of DPSC were obtained from immature third molars. Immunofluorescence assay using STRO-1 marker was performed to confirm the mesenchymal nature of the DPSC. The cells were exposed to TAP, Ca(OH)2, and Ledermix® at concentrations of 0.1 and 1 mg/mL. Cell viability was analyzed using the MTT assay. Results: Significant differences in viability were noted between the cells exposed to the medicaments and those in the control group (p<0.05). Conclusions: All three medicaments decreased the viability of DPSC, with the Ledermix® paste demonstrating the highest toxic effect.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
JournalInternational Journal of Applied Pharmaceutics
Volume11
DOIs
Publication statusPublished - Apr 2019

Keywords

  • Calcium hydroxide
  • Cell viability
  • Dental pulp stem cells
  • Ledermix®
  • Triple antibiotic paste

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