TY - JOUR
T1 - Effect of triple antibiotic paste, calcium hydroxide, ledermix® on viability of pulp mesenchymal stem cells
AU - Prasanti, Emiria Dita
AU - Margono, Anggraini
AU - Djauharie, Nilakesuma
N1 - Publisher Copyright:
© 2019 The Authors.
PY - 2019/4
Y1 - 2019/4
N2 - Objective: The goal of regenerative endodontic therapy is biological healing of the pulp tissue. It involves the disinfection of the canals with irrigants and medicaments. The medicaments that are currently used for this purpose are a triple antibiotic paste (TAP), calcium hydroxide (Ca[OH]2), and Ledermix®, a paste containing demeclocycline and triamcinolone. Therefore, the purpose of this study was to evaluate the effects of TAP, Ca(OH)2, and Ledermix® on the viability of dental pulp stem cells (DPSC). Methods: Primary cultures of DPSC were obtained from immature third molars. Immunofluorescence assay using STRO-1 marker was performed to confirm the mesenchymal nature of the DPSC. The cells were exposed to TAP, Ca(OH)2, and Ledermix® at concentrations of 0.1 and 1 mg/mL. Cell viability was analyzed using the MTT assay. Results: Significant differences in viability were noted between the cells exposed to the medicaments and those in the control group (p<0.05). Conclusions: All three medicaments decreased the viability of DPSC, with the Ledermix® paste demonstrating the highest toxic effect.
AB - Objective: The goal of regenerative endodontic therapy is biological healing of the pulp tissue. It involves the disinfection of the canals with irrigants and medicaments. The medicaments that are currently used for this purpose are a triple antibiotic paste (TAP), calcium hydroxide (Ca[OH]2), and Ledermix®, a paste containing demeclocycline and triamcinolone. Therefore, the purpose of this study was to evaluate the effects of TAP, Ca(OH)2, and Ledermix® on the viability of dental pulp stem cells (DPSC). Methods: Primary cultures of DPSC were obtained from immature third molars. Immunofluorescence assay using STRO-1 marker was performed to confirm the mesenchymal nature of the DPSC. The cells were exposed to TAP, Ca(OH)2, and Ledermix® at concentrations of 0.1 and 1 mg/mL. Cell viability was analyzed using the MTT assay. Results: Significant differences in viability were noted between the cells exposed to the medicaments and those in the control group (p<0.05). Conclusions: All three medicaments decreased the viability of DPSC, with the Ledermix® paste demonstrating the highest toxic effect.
KW - Calcium hydroxide
KW - Cell viability
KW - Dental pulp stem cells
KW - Ledermix®
KW - Triple antibiotic paste
UR - http://www.scopus.com/inward/record.url?scp=85086223421&partnerID=8YFLogxK
U2 - 10.22159/ijap.2019.v11s1.164
DO - 10.22159/ijap.2019.v11s1.164
M3 - Article
AN - SCOPUS:85086223421
SN - 0975-7058
VL - 11
SP - 49
EP - 53
JO - International Journal of Applied Pharmaceutics
JF - International Journal of Applied Pharmaceutics
ER -