Effect of treatment using Carica papaya seed extract with Ag-TiO2 nanocomposite on the mortality of Aedes aegypti larvae

W. S. Adayani, R. Subahar, Fatmawaty, Slamet

Research output: Contribution to journalConference articlepeer-review

3 Citations (Scopus)


The incidence and mortality rate of dengue hemorrhagic fever (DHF) in Indonesia have continued to increase. However, the insecticidal control of the vector of DHF, Aedes aegypti (yellow fever mosquito), has resulted the development of insecticide resistance. The aim of this study was to evaluate the effects, singly and in combination, of two components with proven larvicidal activity, namely, the Ag-TiO2 nanocomposite and Carica papaya seed extract, on the mortality of A. aegypti larvae. This experimental study was categorized into the control and three intervention groups: 1) papaya seed extract at concentrations of 0, 2, 4, 6, 8, and 10 ppm; 2) Ag-TiO2 at concentrations of 0, 5,10,15, 20, and 25 ppm; and 3) mixture of Ag-TiO2 and C. papaya seed extract 50% and 90% lethal concentration. After 24 h exposure, the 50% lethal concentration and the 90% lethal concentration were determined for the Ag-TiO2 nanocomposite (5.19 ppm and 10.87 ppm, respectively) and the C. papaya seed extract (25.98 ppm and 44.30 ppm, respectively). Significant differences were detected among Ag-TiO2 concentrations and mixture-group concentrations (p < 0.05). Significant positive correlations were detected between larval mortality and Ag-TiO2 concentrations and mixture group concentrations (r = 0.812, p = 0.001 and r = 0.343, p < 0.001, respectively). Changes in the visual appearance of dead larvae following treatment with nanocomposites and/or seed extract included damage to the abdominal segments, increased transparency of the abdomen, and reduced brush numbers. Therefore, the addition of the nanocomposite Ag-TiO2 to C. papaya seed extract increased the larvicidal effectiveness of the seed extract against A. aegypti larvae.

Original languageEnglish
Article number032035
JournalJournal of Physics: Conference Series
Issue number3
Publication statusPublished - 7 Sept 2018
Event2nd Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2018 - Depok, West Java, Indonesia
Duration: 18 Jul 201818 Jul 2018


Dive into the research topics of 'Effect of treatment using Carica papaya seed extract with Ag-TiO2 nanocomposite on the mortality of Aedes aegypti larvae'. Together they form a unique fingerprint.

Cite this