TY - GEN
T1 - Effect of toxic components of Scorpaenopsis diabolus poison on HeLa cells through cytotoxic test with MTS assay
AU - Rosari, Trianita Amnina
AU - Perdani, Meka Saima
AU - Hermansyah, Heri
N1 - Publisher Copyright:
© 2024 Author(s).
PY - 2024/2/6
Y1 - 2024/2/6
N2 - Scorpaenopsis diabolus or devil scorpionfish is a species of fish that belong to one of the toxin families in the world, Scorpaenidae. Fish venom from the Scorpaenidae family has bioactive compounds that are reported to have a hemolytic activity that can lyse cells. Extraction was carried out to isolate Scorpaenopsis diabolus toxin using the batch method and purified by FPLC (fast protein liquid chromatography) anion exchange column. The purification results showed that the venom of Scorpaenopsis diabolus had 10 protein peaks. The cytotoxic test was performed on HeLa cells using the MTS Assay method. The cytotoxic test results showed that viability of HeLa cells decreased when exposed to a toxic protein fraction isolated from Scorpaenopsis diabolus. This study aims to isolate protein from Scorpaenopsis diabolus venom and observe the cytotoxic properties of these compounds against HeLa cells which can be used in the future for research on cervical cancer treatment.
AB - Scorpaenopsis diabolus or devil scorpionfish is a species of fish that belong to one of the toxin families in the world, Scorpaenidae. Fish venom from the Scorpaenidae family has bioactive compounds that are reported to have a hemolytic activity that can lyse cells. Extraction was carried out to isolate Scorpaenopsis diabolus toxin using the batch method and purified by FPLC (fast protein liquid chromatography) anion exchange column. The purification results showed that the venom of Scorpaenopsis diabolus had 10 protein peaks. The cytotoxic test was performed on HeLa cells using the MTS Assay method. The cytotoxic test results showed that viability of HeLa cells decreased when exposed to a toxic protein fraction isolated from Scorpaenopsis diabolus. This study aims to isolate protein from Scorpaenopsis diabolus venom and observe the cytotoxic properties of these compounds against HeLa cells which can be used in the future for research on cervical cancer treatment.
UR - http://www.scopus.com/inward/record.url?scp=85185785383&partnerID=8YFLogxK
U2 - 10.1063/5.0161385
DO - 10.1063/5.0161385
M3 - Conference contribution
AN - SCOPUS:85185785383
T3 - AIP Conference Proceedings
BT - AIP Conference Proceedings
A2 - Kusuma, Andyka
A2 - Fatriansyah, Jaka Fajar
A2 - Dhelika, Radon
A2 - Pratama, Mochamad Adhiraga
A2 - Irwansyah, Ridho
A2 - Maknun, Imam Jauhari
A2 - Putra, Wahyuaji Narottama
A2 - Ardi, Romadhani
A2 - Harwahyu, Ruki
A2 - Harahap, Yulia Nurliani
A2 - Lischer, Kenny
PB - American Institute of Physics Inc.
T2 - 17th International Conference on Quality in Research, QiR 2021 in conjunction with the International Tropical Renewable Energy Conference 2021, I-Trec 2021 and the 2nd AUN-SCUD International Conference, CAIC-SIUD
Y2 - 13 October 2021 through 15 October 2021
ER -