Effect of toxic components of Scorpaenopsis diabolus poison on HeLa cells through cytotoxic test with MTS assay

Trianita Amnina Rosari, Meka Saima Perdani, Heri Hermansyah

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Scorpaenopsis diabolus or devil scorpionfish is a species of fish that belong to one of the toxin families in the world, Scorpaenidae. Fish venom from the Scorpaenidae family has bioactive compounds that are reported to have a hemolytic activity that can lyse cells. Extraction was carried out to isolate Scorpaenopsis diabolus toxin using the batch method and purified by FPLC (fast protein liquid chromatography) anion exchange column. The purification results showed that the venom of Scorpaenopsis diabolus had 10 protein peaks. The cytotoxic test was performed on HeLa cells using the MTS Assay method. The cytotoxic test results showed that viability of HeLa cells decreased when exposed to a toxic protein fraction isolated from Scorpaenopsis diabolus. This study aims to isolate protein from Scorpaenopsis diabolus venom and observe the cytotoxic properties of these compounds against HeLa cells which can be used in the future for research on cervical cancer treatment.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
EditorsAndyka Kusuma, Jaka Fajar Fatriansyah, Radon Dhelika, Mochamad Adhiraga Pratama, Ridho Irwansyah, Imam Jauhari Maknun, Wahyuaji Narottama Putra, Romadhani Ardi, Ruki Harwahyu, Yulia Nurliani Harahap, Kenny Lischer
PublisherAmerican Institute of Physics Inc.
Edition1
ISBN (Electronic)9780735446410
DOIs
Publication statusPublished - 6 Feb 2024
Event17th International Conference on Quality in Research, QiR 2021 in conjunction with the International Tropical Renewable Energy Conference 2021, I-Trec 2021 and the 2nd AUN-SCUD International Conference, CAIC-SIUD - Virtual, Online, India
Duration: 13 Oct 202115 Oct 2021

Publication series

NameAIP Conference Proceedings
Number1
Volume2710
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference17th International Conference on Quality in Research, QiR 2021 in conjunction with the International Tropical Renewable Energy Conference 2021, I-Trec 2021 and the 2nd AUN-SCUD International Conference, CAIC-SIUD
Country/TerritoryIndia
CityVirtual, Online
Period13/10/2115/10/21

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