Effect of the combination of ipriflavone and 1.α.-OH-D3 on debilitant bone in growing rats: -Ultrastructural study of endochondral ossification-

Kazushige Ueda, Ikuko Nishida, Bin Xia, Iwan Tofani, Jianguo Wang, Yasuhiro Nishikawa, Mitsutaka Kimura

Research output: Contribution to journalArticlepeer-review

Abstract

Five-week-old male Wistar rats were used to study the effect of dietary therapy with ipriflavone combined with 1α-OH-D3. Ultrastructural alterations in the metaphysis of debilitated tibia were observed in the growing rats. I. Light microscopy findings In the low-calcium diet • standard diet with supplementary ipriflavone and 1α-OH-D3 group, calcification of the chondral matrix and ossification were active, and the tibia grew normally as in the control group. II. Scanning electron microscopy findings In the low-calcium diet • standard diet with supplementary ipriflavone and 1α-OH-D3 group, dense calcospherites, distinct chondral lacunae, regularly running collagen fibers, and distinct border lines were noted. III. Transmission electron microscopy findings In the low-calcium diet • standard diet with supplementary ipriflavone and 1α-OH-D3 group we found that the osteoblasts were active, the ruffled border of osteoclast was decrease, indicated this osteoclast is inactive. In conclusion, insufficient calcium intake during the developmental period resulted in debilitated(3etaphysis tibia, whereas dietary therapy using combined ipriflavone and 1α-OH-D3 promoted recovery.

Original languageEnglish
Pages (from-to)5-15
Number of pages11
Journalpediatric dental journal
Volume14
Issue number1
DOIs
Publication statusPublished - 1 Jan 2010

Keywords

  • 1α-OH-D3
  • Growing rats
  • Ipriflavone
  • Metaphysis tibia

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