TY - GEN
T1 - Effect of temperature shift strategy in the production unsaturated fatty acids from Rhizopus oligosporus
AU - Salehoddin, M. R.
AU - Hafizh, N.
AU - Utami, T. S.
AU - Arbianti, R.
AU - Hermansyah, H.
N1 - Publisher Copyright:
© 2020 Author(s).
PY - 2020/9/3
Y1 - 2020/9/3
N2 - Today the main source of unsaturated fatty acids especially omega-3 is still from marine fish oil. In this research, Rhizopus oligosporus will be used to produce PUFA (polyunsaturated fatty acids). This research used submerged fermentation method and temperature shift strategy to optimize the production of PUFA. In this result, batch culture of R.oligosporus for PUFA production at various temperatures (22, 26, 32, and 37°C). Result showed that R.oligosporus has different optimum temperature for growth and PUFA production, 26°C and 22°C respectively. Based on the result temperature-shift strategy was applied, in which the culture temperature was controlled at 26°C at the first 5 days, and then switched to 22°C for two days. By applying such a temperature-shift strategy, the dry cell weight decreased from 0,55 g to 0,47 g and the concentration of PUFA increased from 19,8% to 22,5%.
AB - Today the main source of unsaturated fatty acids especially omega-3 is still from marine fish oil. In this research, Rhizopus oligosporus will be used to produce PUFA (polyunsaturated fatty acids). This research used submerged fermentation method and temperature shift strategy to optimize the production of PUFA. In this result, batch culture of R.oligosporus for PUFA production at various temperatures (22, 26, 32, and 37°C). Result showed that R.oligosporus has different optimum temperature for growth and PUFA production, 26°C and 22°C respectively. Based on the result temperature-shift strategy was applied, in which the culture temperature was controlled at 26°C at the first 5 days, and then switched to 22°C for two days. By applying such a temperature-shift strategy, the dry cell weight decreased from 0,55 g to 0,47 g and the concentration of PUFA increased from 19,8% to 22,5%.
UR - http://www.scopus.com/inward/record.url?scp=85092020005&partnerID=8YFLogxK
U2 - 10.1063/5.0014698
DO - 10.1063/5.0014698
M3 - Conference contribution
AN - SCOPUS:85092020005
T3 - AIP Conference Proceedings
BT - 4th International Tropical Renewable Energy Conference, i-TREC 2019
A2 - Kusrini, Eny
A2 - Nugraha, I. Gde Dharma
PB - American Institute of Physics Inc.
T2 - 4th International Tropical Renewable Energy Conference 2019, i-TREC 2019
Y2 - 14 August 2019 through 16 August 2019
ER -
