Effect of smear layer deproteinization with chemo-mechanical caries removal agents on sealing performances of self-etch adhesives

Citra Kusumasari, Ahmed Abdou, Antonin Tichy, Takashi Hatayama, Keiichi Hosaka, Richard M. Foxton, Takahiro Wada, Yasunori Sumi, Masatoshi Nakajima, Junji Tagami

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Objectives: To evaluate the sealing performance of self-etch adhesives (SEA) after smear layer deproteinization with chemo-mechanical caries removal agents (CRA) to enamel and dentin cavity walls in comparison with 6% NaOCl smear layer deproteinizing. Methods: Eighty extracted bovine incisors with tapered cylindrical cavities (2.5 mm upper diameter, 1.5 mm lower diameter, 2 mm depth) at the cemento-enamel junction were randomly divided into four pre-treatment groups; no treatment (control), Papacarie (papain enzyme-based CRA), Carisolv (NaOCl-based CRA), and 6% NaOCl. After pretreatment, G-Bond Plus (GBP, one-step SEA) or Clearfil SE Bond 2 (CSE, two-step SEA) was applied to the pre-treated cavity, and then filled with a flowable resin composite (Clearfil Majesty ES Flow). Gap formations at the enamel and dentin lateral walls, and on the dentin cavity floor were investigated using swept-source optical coherence tomography (SS-OCT) after 24-h water storage at 37 °C. Deproteinizing effects of the tested agents on smear layer-covered enamel and dentin were quantified by changes in the amide:phosphate ratio using ATR-FTIR analysis. Results: Papacarie significantly reduced gap formation with both self-etch adhesives at all locations, compared to the control group (p < 0.05). In contrast, increased gap formation was observed with Carisolv and NaOCl. In most groups, CSE exhibited significantly lower gap formation than GBP (p < 0.05). ATR-FTIR revealed a significant decrease in the amide:phosphate ratio for all the deproteinizing agents (p < 0.05). Conclusions: Chemo-mechanical CRAs could effectively remove the organic phase of enamel and dentin smear layer. The papain enzyme-based gel Papacarie could improve the sealing performance of the self-etch adhesives to both enamel and dentin.

Original languageEnglish
Article number103300
JournalJournal of Dentistry
Volume94
DOIs
Publication statusPublished - Mar 2020

Keywords

  • Chemo-mechanical
  • NaOCl-based gel
  • Papain enzyme-based gel
  • Sealing performance
  • Self-etch
  • Smear layer deproteinizing

Fingerprint

Dive into the research topics of 'Effect of smear layer deproteinization with chemo-mechanical caries removal agents on sealing performances of self-etch adhesives'. Together they form a unique fingerprint.

Cite this