Effect Of Rotation and Reciprocating Single File System on Enterococcus Faecalis in Debris Apical Extrusion

Dwi Arniawaty, Dewa Ayu Nyoman Putri Artiningsih, Endang Suprastiwi, Kamizar, Stefani Astari

Research output: Contribution to journalArticlepeer-review


Objective: The preparation technique, file design, and irrigation technique influence the risk of debris and the bacterial extrusion during root canal preparation, whichtcan lead to inflammation in the periapical tissue. Thus, this study sought to evaluate and compare the effects of continuous-rotation and reciprocating single-file application on debris extrusion, and the presence of Enterococcus faecalis. Methods: Single roots were randomly contaminated with E. faecalis bacterial isolates into the root canal of 32 mandibular premolars, then divided into two groups. Continuous-rotation single file and reciprocating single file Reciproc Blue were used to create root canals (VDW Dental, Germany). Data on debris and bacterial extrusions is collected using the Myers and Montogomery models. Weighing the trash tubes before and after instrumentation allowed us to detect extrusions. The presence of E. faecalis in the collected trash was determined by the development of blue-green colonies on CHROMagar. The Mann–Whitney U nonparametric test and categorical comparative test..were applied..to elucidate significant..differences..among the groups. Results: No..significant..differences between debris extrusion (p = 0.513) and the number of E. faecalis organisms (p = 0.479) were seen between the continuous rotation and reciprocating single files. Conclusion: Both continuous-rotation and reciprocating single-file systems were linked to debris and E. faecalis extrusion in this investigation, although there were no significant differences in debris weight or E. faecalis extrusion between the two instruments.
Original languageEnglish
Article number13
Pages (from-to)836-842
Number of pages7
JournalInternational Journal of Pharmaceutical Research
Issue number3
Publication statusPublished - 9 Apr 2021


  • Enterococcus faecalis
  • periapical tissue
  • root canal preparation


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