Effect of reuterin on dual-species biofilm in vitro of Streptococcus mutans and Veillonella parvula

Armelia Sari Widyarman, Citra Fragrantia Theodorea

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8 Citations (Scopus)


Probiotics have potential to restore homeostatis that may not be achieved with conventional treatment, by more effectively addressing the host-agent interaction. This study has been conducted to analyze the inhibitory effects of reuterin isolate Lactobacillus reuteri on the dual-species biofilm formation of S. mutans and V. parvula in-vitro. V. parvula ATCC 10790T and S. mutans ATCC 25175T were used in biofilm formation. Reuterin isolated from L. reuteri LC382415 (1.5×1010CFU/ml) were suspended in 5 ml of MRS broth containing glycerol (300mM) and incubated for 3h, at 37°C, anaerobically and confirmed by using SDS-PAGE and dot blot methods. 96-well flat-bottom microtiter plates biofilm assay were used to analyze the reuterin effect on dual-species biofilm. Bacterial suspensions were treated with different reuterin concentrations and biofilm inhibitory effect was observed periodically after 15 min, 3 h, 6 h, and 24 h incubation time. Data statistically analyzed by One-way ANOVA and level of significance set at p < 0.05. 52 kDa band visualized on SDS-PAGE agar confirmed the presence of reuterin. The reuterin inhibitory effect on dual-species biofilm of S. mutans and V. parvula were found in this study. Reuterin had the strongest activity on dual-species biofilm with the 100% concentration for 24h period. Meanwhile, the percentage reduction of biofilm after treatment with 50% concentration of reuterin for 6h could be reach until 91% reduction (p < 0.05). This study is the first report to demonstrate that reuterin isolated from L. reuteri might be implicated to prevent the biofilm formation at early stages. Further studies are needed to explore this result with other oral pathogen.

Original languageEnglish
Pages (from-to)77-83
Number of pages7
JournalJournal of International Dental and Medical Research
Issue number1
Publication statusPublished - 2019


  • Oral biofilms
  • Reuterin
  • Streptococcus mutans
  • Veillonella parvula


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