Effect of Platelet Rich Plasma Incorporated to Autologous Bone Graft on Collagen Production in vivo

Platelet-rich plasma (PRP) has become a valuable adjunct to several procedures in oral and maxillofacial surgery as it can promote bone tissue regeneration without the risk of toxicity and immunogenic reactions. Moreover, collagen plays an important role in wound healing. To investigate the effect of PRP incorporated to nonvascularized autogenous bone grafts on collagen production in vivo using an Ovis aries critical-sized mandibular bone defect model. The extracted PRP was mixed with autogenous bone mills and was augmented to the mandibular bone defect model. The volume of collagen produced at 3 and 6 weeks was evaluated. Masson’s trichrome was used for histological examination, and the volume of collagen produced was quantified using ImageJ. The PRP group had a significantly higher collagen volume at 3 and 6 weeks than the control group, and the volume of collagen produced at 6 weeks was higher than that produced at 3 weeks in the PRP group. However, the results did not significantly differ. Incorporating PRP to autogenous bone grafts increases collagen production at 3 and 6 weeks, which can facilitate mandibular bone healing in sheep.